Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2056 | 3' | -55 | NC_001348.1 | + | 105721 | 0.65 | 0.913688 |
Target: 5'- ---aGCGcgGCACCCaggccccaUCGGUuuGCUGCg -3' miRNA: 3'- gauaCGCuaCGUGGGa-------AGUCG--CGGCG- -5' |
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2056 | 3' | -55 | NC_001348.1 | + | 51362 | 0.65 | 0.913688 |
Target: 5'- ---gGCGGgUGCAUaucUCAGCGCCGg -3' miRNA: 3'- gauaCGCU-ACGUGggaAGUCGCGGCg -5' |
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2056 | 3' | -55 | NC_001348.1 | + | 124071 | 0.65 | 0.913688 |
Target: 5'- ---aGCGcgGCACCCaggccccaUCGGUuuGCUGCg -3' miRNA: 3'- gauaCGCuaCGUGGGa-------AGUCG--CGGCG- -5' |
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2056 | 3' | -55 | NC_001348.1 | + | 84164 | 0.66 | 0.907534 |
Target: 5'- --cUGCGAUGCAUCC--CGGgGUaCGCg -3' miRNA: 3'- gauACGCUACGUGGGaaGUCgCG-GCG- -5' |
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2056 | 3' | -55 | NC_001348.1 | + | 40052 | 0.66 | 0.901133 |
Target: 5'- -cGUGCGuugGUuuuACCCcaUUCGGCGUgGCg -3' miRNA: 3'- gaUACGCua-CG---UGGG--AAGUCGCGgCG- -5' |
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2056 | 3' | -55 | NC_001348.1 | + | 80453 | 0.67 | 0.873122 |
Target: 5'- ---cGCGcUGCACCCguagCGGUGUacaGCg -3' miRNA: 3'- gauaCGCuACGUGGGaa--GUCGCGg--CG- -5' |
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2056 | 3' | -55 | NC_001348.1 | + | 105371 | 0.67 | 0.857737 |
Target: 5'- --cUGCGGUGCGCCgCUgucGCcCCGCc -3' miRNA: 3'- gauACGCUACGUGG-GAaguCGcGGCG- -5' |
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2056 | 3' | -55 | NC_001348.1 | + | 124421 | 0.67 | 0.857737 |
Target: 5'- --cUGCGGUGCGCCgCUgucGCcCCGCc -3' miRNA: 3'- gauACGCUACGUGG-GAaguCGcGGCG- -5' |
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2056 | 3' | -55 | NC_001348.1 | + | 118095 | 0.73 | 0.532779 |
Target: 5'- ---gGCGAaGUACCgUUCGGCGUCGUu -3' miRNA: 3'- gauaCGCUaCGUGGgAAGUCGCGGCG- -5' |
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2056 | 3' | -55 | NC_001348.1 | + | 111697 | 0.73 | 0.532779 |
Target: 5'- ---gGCGAaGUACCgUUCGGCGUCGUu -3' miRNA: 3'- gauaCGCUaCGUGGgAAGUCGCGGCG- -5' |
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2056 | 3' | -55 | NC_001348.1 | + | 2228 | 1.13 | 0.001518 |
Target: 5'- cCUAUGCGAUGCACCCUUCAGCGCCGCa -3' miRNA: 3'- -GAUACGCUACGUGGGAAGUCGCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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