Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20568 | 5' | -56.1 | NC_004688.1 | + | 39818 | 0.66 | 0.830402 |
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Target: 5'- cGGuGCCGugGUUGUCGCaGUguuugGGUGCGg -3' miRNA: 3'- cUC-CGGCugCGAUAGCG-CG-----UCAUGUa -5' |
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| 20568 | 5' | -56.1 | NC_004688.1 | + | 14907 | 0.66 | 0.830402 |
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Target: 5'- cGAGGCCGugGagcuUC-CGCAGgccGCAUg -3' miRNA: 3'- -CUCCGGCugCgau-AGcGCGUCa--UGUA- -5' |
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| 20568 | 5' | -56.1 | NC_004688.1 | + | 9049 | 0.67 | 0.784862 |
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Target: 5'- cAGGCCGACGCUggugGUCGgGUug-GCGc -3' miRNA: 3'- cUCCGGCUGCGA----UAGCgCGucaUGUa -5' |
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| 20568 | 5' | -56.1 | NC_004688.1 | + | 20308 | 0.67 | 0.775274 |
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Target: 5'- cGAGGCCGAcCGCgggGUCG-GCcagguuGUGCAc -3' miRNA: 3'- -CUCCGGCU-GCGa--UAGCgCGu-----CAUGUa -5' |
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| 20568 | 5' | -56.1 | NC_004688.1 | + | 11402 | 0.67 | 0.775274 |
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Target: 5'- aAGGUCGGUGCcGUCaGCGCGGUGCc- -3' miRNA: 3'- cUCCGGCUGCGaUAG-CGCGUCAUGua -5' |
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| 20568 | 5' | -56.1 | NC_004688.1 | + | 106054 | 0.67 | 0.76555 |
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Target: 5'- --cGCCGGCGCUA---UGCAGUACGUc -3' miRNA: 3'- cucCGGCUGCGAUagcGCGUCAUGUA- -5' |
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| 20568 | 5' | -56.1 | NC_004688.1 | + | 86317 | 0.68 | 0.715228 |
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Target: 5'- -cGGCCGugGCccgCGCGUcccccAGUGCGg -3' miRNA: 3'- cuCCGGCugCGauaGCGCG-----UCAUGUa -5' |
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| 20568 | 5' | -56.1 | NC_004688.1 | + | 50643 | 0.68 | 0.682994 |
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Target: 5'- -cGGuuGGCGCUGUCGUaucugagGgAGUACGUg -3' miRNA: 3'- cuCCggCUGCGAUAGCG-------CgUCAUGUA- -5' |
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| 20568 | 5' | -56.1 | NC_004688.1 | + | 102436 | 1.05 | 0.003239 |
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Target: 5'- uGAGGCCGACGCUAUCGCGCAGUACAUc -3' miRNA: 3'- -CUCCGGCUGCGAUAGCGCGUCAUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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