Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2057 | 3' | -51.9 | NC_001348.1 | + | 47521 | 0.66 | 0.965616 |
Target: 5'- aAUACCGCagguaaAC-GGUGUggGGAAGCAAa -3' miRNA: 3'- -UAUGGCGg-----UGaCCGCAa-CCUUUGUUg -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 62947 | 0.66 | 0.963525 |
Target: 5'- cAUACCGCCccccacgagcaugaaACUGGuCGcgUGGuAGACGAUc -3' miRNA: 3'- -UAUGGCGG---------------UGACC-GCa-ACC-UUUGUUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 772 | 0.66 | 0.962083 |
Target: 5'- -aACCGCCGgUGGUcUUGuuuGAACGACg -3' miRNA: 3'- uaUGGCGGUgACCGcAACc--UUUGUUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 85110 | 0.66 | 0.962083 |
Target: 5'- gAUGCgGCgGCaaUGGCGUUuGGAAAaacCAACg -3' miRNA: 3'- -UAUGgCGgUG--ACCGCAA-CCUUU---GUUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 111216 | 0.67 | 0.940158 |
Target: 5'- -cACCGCguUgGGCGagggggguguagaUUGGAAGCGACg -3' miRNA: 3'- uaUGGCGguGaCCGC-------------AACCUUUGUUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 118576 | 0.67 | 0.940158 |
Target: 5'- -cACCGCguUgGGCGagggggguguagaUUGGAAGCGACg -3' miRNA: 3'- uaUGGCGguGaCCGC-------------AACCUUUGUUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 106862 | 0.68 | 0.930249 |
Target: 5'- gAUAcCCGCCucUUGGCcUUGGAAACcACa -3' miRNA: 3'- -UAU-GGCGGu-GACCGcAACCUUUGuUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 122929 | 0.68 | 0.930249 |
Target: 5'- gAUAcCCGCCucUUGGCcUUGGAAACcACa -3' miRNA: 3'- -UAU-GGCGGu-GACCGcAACCUUUGuUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 54708 | 0.68 | 0.925219 |
Target: 5'- uUACUGuCCACUGGacuguaagcugaaugGUUGGAGACAc- -3' miRNA: 3'- uAUGGC-GGUGACCg--------------CAACCUUUGUug -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 124185 | 0.68 | 0.906234 |
Target: 5'- -gGCCGCgGagacGGUGUUGGAggggAGCGACg -3' miRNA: 3'- uaUGGCGgUga--CCGCAACCU----UUGUUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 105607 | 0.68 | 0.906234 |
Target: 5'- -gGCCGCgGagacGGUGUUGGAggggAGCGACg -3' miRNA: 3'- uaUGGCGgUga--CCGCAACCU----UUGUUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 3510 | 0.69 | 0.868784 |
Target: 5'- cGUACCGagucaauggucaCGCUGGCucccgcggUGGAGACGACu -3' miRNA: 3'- -UAUGGCg-----------GUGACCGca------ACCUUUGUUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 42800 | 0.69 | 0.862431 |
Target: 5'- --cCCGCCGCguUGGCGUUuucGGGAucauccccGCAACa -3' miRNA: 3'- uauGGCGGUG--ACCGCAA---CCUU--------UGUUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 123467 | 0.7 | 0.85428 |
Target: 5'- -cGCCgGUUGCUGGUGUUGGAcGCggUg -3' miRNA: 3'- uaUGG-CGGUGACCGCAACCUuUGuuG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 106737 | 0.7 | 0.85428 |
Target: 5'- -aAUCGCCGCUcggcguccgGGCGgaGGAGAcCGACg -3' miRNA: 3'- uaUGGCGGUGA---------CCGCaaCCUUU-GUUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 106325 | 0.7 | 0.85428 |
Target: 5'- -cGCCgGUUGCUGGUGUUGGAcGCggUg -3' miRNA: 3'- uaUGG-CGGUGACCGCAACCUuUGuuG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 123055 | 0.7 | 0.85428 |
Target: 5'- -aAUCGCCGCUcggcguccgGGCGgaGGAGAcCGACg -3' miRNA: 3'- uaUGGCGGUGA---------CCGCaaCCUUU-GUUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 81352 | 0.71 | 0.810313 |
Target: 5'- gGUACagagaGaCCACUGGCGUUGGuucccgcuAUAACg -3' miRNA: 3'- -UAUGg----C-GGUGACCGCAACCuu------UGUUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 16529 | 0.73 | 0.696341 |
Target: 5'- uUACCGCCGuCUccaaagccucgccgGGCGUUGGAAuaaGACa -3' miRNA: 3'- uAUGGCGGU-GA--------------CCGCAACCUUug-UUG- -5' |
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2057 | 3' | -51.9 | NC_001348.1 | + | 33168 | 0.75 | 0.591689 |
Target: 5'- gAUACCaacaGCCugaagauCUGGCGUUGGAuaaaAACGACa -3' miRNA: 3'- -UAUGG----CGGu------GACCGCAACCU----UUGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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