Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2057 | 5' | -55.3 | NC_001348.1 | + | 703 | 0.66 | 0.871869 |
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Target: 5'- uUGCauuUUCAguUGCGCgGACGCCu -3' miRNA: 3'- gACGguuGAGUguACGUGgCUGCGGu -5' |
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| 2057 | 5' | -55.3 | NC_001348.1 | + | 50375 | 0.66 | 0.864244 |
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Target: 5'- -gGUCGACUaa-GUGUACCGACGUgAa -3' miRNA: 3'- gaCGGUUGAgugUACGUGGCUGCGgU- -5' |
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| 2057 | 5' | -55.3 | NC_001348.1 | + | 71761 | 0.67 | 0.840075 |
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Target: 5'- -aGCUGGCUUACGUGCGagaUGGCGUUAu -3' miRNA: 3'- gaCGGUUGAGUGUACGUg--GCUGCGGU- -5' |
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| 2057 | 5' | -55.3 | NC_001348.1 | + | 91067 | 0.67 | 0.822957 |
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Target: 5'- gUGUgGGCgUUACAUGCGCUGACuGCUg -3' miRNA: 3'- gACGgUUG-AGUGUACGUGGCUG-CGGu -5' |
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| 2057 | 5' | -55.3 | NC_001348.1 | + | 100451 | 0.68 | 0.786601 |
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Target: 5'- gUGCUAACaCACGcGCAuCCGuCGCCAu -3' miRNA: 3'- gACGGUUGaGUGUaCGU-GGCuGCGGU- -5' |
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| 2057 | 5' | -55.3 | NC_001348.1 | + | 123427 | 0.69 | 0.757768 |
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Target: 5'- -gGCCGucgaguaucuagGCUCGCGguuggccucUGC-CCGGCGCCGg -3' miRNA: 3'- gaCGGU------------UGAGUGU---------ACGuGGCUGCGGU- -5' |
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| 2057 | 5' | -55.3 | NC_001348.1 | + | 106365 | 0.69 | 0.757768 |
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Target: 5'- -gGCCGucgaguaucuagGCUCGCGguuggccucUGC-CCGGCGCCGg -3' miRNA: 3'- gaCGGU------------UGAGUGU---------ACGuGGCUGCGGU- -5' |
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| 2057 | 5' | -55.3 | NC_001348.1 | + | 39278 | 0.7 | 0.676467 |
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Target: 5'- aUGUCAAC-CACAgaCGCCGAUGCCu -3' miRNA: 3'- gACGGUUGaGUGUacGUGGCUGCGGu -5' |
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| 2057 | 5' | -55.3 | NC_001348.1 | + | 2449 | 1.09 | 0.002583 |
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Target: 5'- cCUGCCAACUCACAUGCACCGACGCCAu -3' miRNA: 3'- -GACGGUUGAGUGUACGUGGCUGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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