Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20571 | 3' | -51.6 | NC_004688.1 | + | 42322 | 0.66 | 0.967998 |
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Target: 5'- cGCgguCGGUGAACugcaGgaCGUCGGGCGu -3' miRNA: 3'- aCGaguGCUACUUG----CaaGUAGCCCGU- -5' |
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| 20571 | 3' | -51.6 | NC_004688.1 | + | 25561 | 0.67 | 0.961064 |
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Target: 5'- cGCauUCGCGAUGGggGCGUUCAUCuuGCc -3' miRNA: 3'- aCG--AGUGCUACU--UGCAAGUAGccCGu -5' |
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| 20571 | 3' | -51.6 | NC_004688.1 | + | 95748 | 0.67 | 0.948819 |
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Target: 5'- gGUUCACGAcgGaAGCGgaCGcCGGGCAg -3' miRNA: 3'- aCGAGUGCUa-C-UUGCaaGUaGCCCGU- -5' |
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| 20571 | 3' | -51.6 | NC_004688.1 | + | 17447 | 0.68 | 0.923227 |
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Target: 5'- cGCUUGCGGccgggUGAACGaa-GUCGGGUg -3' miRNA: 3'- aCGAGUGCU-----ACUUGCaagUAGCCCGu -5' |
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| 20571 | 3' | -51.6 | NC_004688.1 | + | 109046 | 0.69 | 0.883087 |
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Target: 5'- aUGCUCGCacUGAuCGUUUgggugcugucaugAUCGGGCAg -3' miRNA: 3'- -ACGAGUGcuACUuGCAAG-------------UAGCCCGU- -5' |
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| 20571 | 3' | -51.6 | NC_004688.1 | + | 103646 | 0.76 | 0.516697 |
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Target: 5'- aGCUCGUGGUGAGCG-UCAaUGGGCAa -3' miRNA: 3'- aCGAGUGCUACUUGCaAGUaGCCCGU- -5' |
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| 20571 | 3' | -51.6 | NC_004688.1 | + | 107149 | 1.1 | 0.00467 |
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Target: 5'- aUGCUCACGAUGAACGUUCAUCGGGCAa -3' miRNA: 3'- -ACGAGUGCUACUUGCAAGUAGCCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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