Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20579 | 3' | -52.4 | NC_004688.1 | + | 7422 | 0.66 | 0.955723 |
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Target: 5'- --gGCGAGUACGGCaAUcGC-UGGACc -3' miRNA: 3'- cuaUGCUCAUGUCGcUGuCGcACCUG- -5' |
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| 20579 | 3' | -52.4 | NC_004688.1 | + | 59689 | 0.66 | 0.95156 |
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Target: 5'- -cUGCGGGUGguCGGCGGCAGgCGacGGCg -3' miRNA: 3'- cuAUGCUCAU--GUCGCUGUC-GCacCUG- -5' |
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| 20579 | 3' | -52.4 | NC_004688.1 | + | 106850 | 0.66 | 0.946687 |
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Target: 5'- --cACGAGUAgugaaccgccgucCAGaCGGCGGCGUGcACg -3' miRNA: 3'- cuaUGCUCAU-------------GUC-GCUGUCGCACcUG- -5' |
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| 20579 | 3' | -52.4 | NC_004688.1 | + | 74037 | 0.66 | 0.942468 |
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Target: 5'- aGAUACGAG-GCcGCGACAGCGc---- -3' miRNA: 3'- -CUAUGCUCaUGuCGCUGUCGCaccug -5' |
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| 20579 | 3' | -52.4 | NC_004688.1 | + | 36651 | 0.67 | 0.921145 |
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Target: 5'- aAUGCGuGgacauuCAGCGGCGGUGaGGGCu -3' miRNA: 3'- cUAUGCuCau----GUCGCUGUCGCaCCUG- -5' |
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| 20579 | 3' | -52.4 | NC_004688.1 | + | 35637 | 0.68 | 0.888591 |
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Target: 5'- --gGCGAGcACGGUGGCAcccGCGcGGACc -3' miRNA: 3'- cuaUGCUCaUGUCGCUGU---CGCaCCUG- -5' |
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| 20579 | 3' | -52.4 | NC_004688.1 | + | 86629 | 0.69 | 0.869169 |
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Target: 5'- ---uCGGGUucCAGCGACGGCGUccgucgcgcccguggGGACc -3' miRNA: 3'- cuauGCUCAu-GUCGCUGUCGCA---------------CCUG- -5' |
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| 20579 | 3' | -52.4 | NC_004688.1 | + | 53277 | 0.7 | 0.8239 |
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Target: 5'- --aGCGGGcAUGGCGACAGCGguaauagaaaGGGCg -3' miRNA: 3'- cuaUGCUCaUGUCGCUGUCGCa---------CCUG- -5' |
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| 20579 | 3' | -52.4 | NC_004688.1 | + | 92994 | 0.72 | 0.726454 |
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Target: 5'- --cGCGAG-ACAguGCGGCGGCGaGGGCg -3' miRNA: 3'- cuaUGCUCaUGU--CGCUGUCGCaCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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