Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2059 | 5' | -51.8 | NC_001348.1 | + | 123912 | 0.66 | 0.968542 |
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Target: 5'- aGGGGGACgcGGGUCUUGgggcccgccgggUACACACGGu -3' miRNA: 3'- aUUCCCUG--UCUGGAACa-----------GUGUGUGCC- -5' |
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| 2059 | 5' | -51.8 | NC_001348.1 | + | 72121 | 0.67 | 0.962487 |
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Target: 5'- cGAGGGACAucuaaaucGugUUg--CACGCGCGGc -3' miRNA: 3'- aUUCCCUGU--------CugGAacaGUGUGUGCC- -5' |
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| 2059 | 5' | -51.8 | NC_001348.1 | + | 105879 | 0.67 | 0.950677 |
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Target: 5'- aGGGGGACgcGGGUCUUGgggCcCGCGCGGg -3' miRNA: 3'- aUUCCCUG--UCUGGAACa--GuGUGUGCC- -5' |
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| 2059 | 5' | -51.8 | NC_001348.1 | + | 42174 | 0.67 | 0.950677 |
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Target: 5'- cGAGGGACc-GCCUUGggagCACugGCa- -3' miRNA: 3'- aUUCCCUGucUGGAACa---GUGugUGcc -5' |
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| 2059 | 5' | -51.8 | NC_001348.1 | + | 42476 | 0.68 | 0.943462 |
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Target: 5'- -uGGGGGCGGgguagacuguggcacGCCUUGggcCGCGgGCGGu -3' miRNA: 3'- auUCCCUGUC---------------UGGAACa--GUGUgUGCC- -5' |
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| 2059 | 5' | -51.8 | NC_001348.1 | + | 47272 | 0.68 | 0.936612 |
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Target: 5'- -cAGGcGGCAGGCUUUGUUuguAUACACGc -3' miRNA: 3'- auUCC-CUGUCUGGAACAG---UGUGUGCc -5' |
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| 2059 | 5' | -51.8 | NC_001348.1 | + | 10911 | 0.69 | 0.904131 |
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Target: 5'- aAAGGGGCAGGCgUguauaagagggccccUGUUuaAUACGCGGu -3' miRNA: 3'- aUUCCCUGUCUGgA---------------ACAG--UGUGUGCC- -5' |
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| 2059 | 5' | -51.8 | NC_001348.1 | + | 112632 | 0.7 | 0.865301 |
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Target: 5'- gGAGGGugAGGCCgUGgccUUACugAUGGa -3' miRNA: 3'- aUUCCCugUCUGGaAC---AGUGugUGCC- -5' |
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| 2059 | 5' | -51.8 | NC_001348.1 | + | 42577 | 1.1 | 0.004785 |
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Target: 5'- gUAAGGGACAGACCUUGUCACACACGGg -3' miRNA: 3'- -AUUCCCUGUCUGGAACAGUGUGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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