Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2060 | 3' | -48.6 | NC_001348.1 | + | 91591 | 0.66 | 0.998424 |
Target: 5'- -gGGACGCgGgccggauaucuACCGUCU-UCAAacaGCCUg -3' miRNA: 3'- uaUCUGCGgU-----------UGGCAGAaAGUU---UGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 42830 | 0.66 | 0.998424 |
Target: 5'- -cAGAgCGCCGAUCaGUCUgcUUUAcaacAGCCCg -3' miRNA: 3'- uaUCU-GCGGUUGG-CAGA--AAGU----UUGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 100919 | 0.66 | 0.998424 |
Target: 5'- uUAGAauaCAAUCGUCUUUUAAAUUCa -3' miRNA: 3'- uAUCUgcgGUUGGCAGAAAGUUUGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 16970 | 0.66 | 0.998096 |
Target: 5'- uGUGGACGCCAGgguaUGUCgccaucUCAAACUUc -3' miRNA: 3'- -UAUCUGCGGUUg---GCAGaa----AGUUUGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 46266 | 0.66 | 0.997712 |
Target: 5'- -cAGaACGCCAuGCCG-CcgUCAAACCa -3' miRNA: 3'- uaUC-UGCGGU-UGGCaGaaAGUUUGGg -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 8990 | 0.66 | 0.997712 |
Target: 5'- gGUGGAUGUUAcuacggaugauAUUGUCgaUCAAACCCu -3' miRNA: 3'- -UAUCUGCGGU-----------UGGCAGaaAGUUUGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 106565 | 0.66 | 0.997266 |
Target: 5'- -gGGugGaCCaAACCGUCggUCuccuccGCCCg -3' miRNA: 3'- uaUCugC-GG-UUGGCAGaaAGuu----UGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 79089 | 0.66 | 0.997266 |
Target: 5'- -aGGACGCCAucaaaggaACCGUUauugaUCGGAUCUg -3' miRNA: 3'- uaUCUGCGGU--------UGGCAGaa---AGUUUGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 123227 | 0.66 | 0.997266 |
Target: 5'- -gGGugGaCCaAACCGUCggUCuccuccGCCCg -3' miRNA: 3'- uaUCugC-GG-UUGGCAGaaAGuu----UGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 91456 | 0.66 | 0.99675 |
Target: 5'- --uGugGCCAguaACCGguaUUCAGGCCUu -3' miRNA: 3'- uauCugCGGU---UGGCagaAAGUUUGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 107342 | 0.67 | 0.995474 |
Target: 5'- --cGugGCUAugaGCCGUCgauacgacCGGGCCCa -3' miRNA: 3'- uauCugCGGU---UGGCAGaaa-----GUUUGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 105034 | 0.67 | 0.995474 |
Target: 5'- -aGGAgGCCAGCCcUCUcgCGGcCCCc -3' miRNA: 3'- uaUCUgCGGUUGGcAGAaaGUUuGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 122450 | 0.67 | 0.995474 |
Target: 5'- --cGugGCUAugaGCCGUCgauacgacCGGGCCCa -3' miRNA: 3'- uauCugCGGU---UGGCAGaaa-----GUUUGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 47824 | 0.67 | 0.994698 |
Target: 5'- aAUAGACggGCCAuGCCGUCUgggauGGCCg -3' miRNA: 3'- -UAUCUG--CGGU-UGGCAGAaagu-UUGGg -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 48348 | 0.68 | 0.9858 |
Target: 5'- -----aGCCAACCaGUCUUUUAAaagaacACCCa -3' miRNA: 3'- uaucugCGGUUGG-CAGAAAGUU------UGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 8040 | 0.7 | 0.972718 |
Target: 5'- uAUGGAUGCUcGCCGUUUugguauauuguucgaUUUGGACCCu -3' miRNA: 3'- -UAUCUGCGGuUGGCAGA---------------AAGUUUGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 508 | 0.71 | 0.949056 |
Target: 5'- cGUGGuCGCCAuuguuGCCGUUUUUCccgagGAACCUc -3' miRNA: 3'- -UAUCuGCGGU-----UGGCAGAAAG-----UUUGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 123567 | 0.72 | 0.93324 |
Target: 5'- -cGGACGCCcaGGCCGUCguccgauggcCAGACUCg -3' miRNA: 3'- uaUCUGCGG--UUGGCAGaaa-------GUUUGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 106225 | 0.72 | 0.93324 |
Target: 5'- -cGGACGCCcaGGCCGUCguccgauggcCAGACUCg -3' miRNA: 3'- uaUCUGCGG--UUGGCAGaaa-------GUUUGGG- -5' |
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2060 | 3' | -48.6 | NC_001348.1 | + | 51408 | 0.72 | 0.928851 |
Target: 5'- --cGAUGUUAACCGUCUggUAcccGACCCa -3' miRNA: 3'- uauCUGCGGUUGGCAGAaaGU---UUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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