Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20601 | 5' | -56.3 | NC_004688.1 | + | 20642 | 0.66 | 0.848927 |
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Target: 5'- aGGCGCgGCGGg-CGGUcgucGGUCCaGUUg- -3' miRNA: 3'- -CCGCGaUGCCgaGCCA----CCAGG-CAAaa -5' |
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| 20601 | 5' | -56.3 | NC_004688.1 | + | 64485 | 0.66 | 0.83231 |
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Target: 5'- aGUGCUACGGUUCGGccGGaugcaacgUCCGa--- -3' miRNA: 3'- cCGCGAUGCCGAGCCa-CC--------AGGCaaaa -5' |
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| 20601 | 5' | -56.3 | NC_004688.1 | + | 86612 | 0.66 | 0.830605 |
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Target: 5'- aGCGCUGCGGCccgcguUCGGguuccagcgacgGcGUCCGUc-- -3' miRNA: 3'- cCGCGAUGCCG------AGCCa-----------C-CAGGCAaaa -5' |
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| 20601 | 5' | -56.3 | NC_004688.1 | + | 97791 | 0.66 | 0.805983 |
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Target: 5'- cGGCGCcgugACGuGUgaUCGGUGGcgucUCCGUg-- -3' miRNA: 3'- -CCGCGa---UGC-CG--AGCCACC----AGGCAaaa -5' |
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| 20601 | 5' | -56.3 | NC_004688.1 | + | 20974 | 0.67 | 0.787596 |
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Target: 5'- gGGUGCgGCGGCUCgccgccuggccGG-GGUCCGc--- -3' miRNA: 3'- -CCGCGaUGCCGAG-----------CCaCCAGGCaaaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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