miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20603 3' -54.1 NC_004688.1 + 4187 0.66 0.932005
Target:  5'- aGCAUCCGCugaCACGUCGUgcgcauUCgCGGa-- -3'
miRNA:   3'- -CGUAGGCGcg-GUGCAGCA------AG-GUCaac -5'
20603 3' -54.1 NC_004688.1 + 68570 0.66 0.932005
Target:  5'- gGCGUCCG-GCCGcCGUaCGcuUUCCAGa-- -3'
miRNA:   3'- -CGUAGGCgCGGU-GCA-GC--AAGGUCaac -5'
20603 3' -54.1 NC_004688.1 + 95658 0.66 0.921043
Target:  5'- gGCG-CCGCGCCGCGgcacaugaaaGgugUCCAGg-- -3'
miRNA:   3'- -CGUaGGCGCGGUGCag--------Ca--AGGUCaac -5'
20603 3' -54.1 NC_004688.1 + 100389 0.69 0.799422
Target:  5'- cGCAUacccacCCGUGCCAC--CGUUCCGGUc- -3'
miRNA:   3'- -CGUA------GGCGCGGUGcaGCAAGGUCAac -5'
20603 3' -54.1 NC_004688.1 + 57126 0.7 0.761379
Target:  5'- uGCGguagCCGUGCUugGUgGUggcuucCCGGUUGg -3'
miRNA:   3'- -CGUa---GGCGCGGugCAgCAa-----GGUCAAC- -5'
20603 3' -54.1 NC_004688.1 + 42147 0.7 0.731541
Target:  5'- uCAUCCGCGCCGCaa-GUUCCGa--- -3'
miRNA:   3'- cGUAGGCGCGGUGcagCAAGGUcaac -5'
20603 3' -54.1 NC_004688.1 + 90110 0.71 0.700885
Target:  5'- aCGUCCGCGUCgGCcUUGUUCCAGcUGg -3'
miRNA:   3'- cGUAGGCGCGG-UGcAGCAAGGUCaAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.