Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20603 | 3' | -54.1 | NC_004688.1 | + | 4187 | 0.66 | 0.932005 |
Target: 5'- aGCAUCCGCugaCACGUCGUgcgcauUCgCGGa-- -3' miRNA: 3'- -CGUAGGCGcg-GUGCAGCA------AG-GUCaac -5' |
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20603 | 3' | -54.1 | NC_004688.1 | + | 68570 | 0.66 | 0.932005 |
Target: 5'- gGCGUCCG-GCCGcCGUaCGcuUUCCAGa-- -3' miRNA: 3'- -CGUAGGCgCGGU-GCA-GC--AAGGUCaac -5' |
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20603 | 3' | -54.1 | NC_004688.1 | + | 95658 | 0.66 | 0.921043 |
Target: 5'- gGCG-CCGCGCCGCGgcacaugaaaGgugUCCAGg-- -3' miRNA: 3'- -CGUaGGCGCGGUGCag--------Ca--AGGUCaac -5' |
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20603 | 3' | -54.1 | NC_004688.1 | + | 100389 | 0.69 | 0.799422 |
Target: 5'- cGCAUacccacCCGUGCCAC--CGUUCCGGUc- -3' miRNA: 3'- -CGUA------GGCGCGGUGcaGCAAGGUCAac -5' |
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20603 | 3' | -54.1 | NC_004688.1 | + | 57126 | 0.7 | 0.761379 |
Target: 5'- uGCGguagCCGUGCUugGUgGUggcuucCCGGUUGg -3' miRNA: 3'- -CGUa---GGCGCGGugCAgCAa-----GGUCAAC- -5' |
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20603 | 3' | -54.1 | NC_004688.1 | + | 42147 | 0.7 | 0.731541 |
Target: 5'- uCAUCCGCGCCGCaa-GUUCCGa--- -3' miRNA: 3'- cGUAGGCGCGGUGcagCAAGGUcaac -5' |
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20603 | 3' | -54.1 | NC_004688.1 | + | 90110 | 0.71 | 0.700885 |
Target: 5'- aCGUCCGCGUCgGCcUUGUUCCAGcUGg -3' miRNA: 3'- cGUAGGCGCGG-UGcAGCAAGGUCaAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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