Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20606 | 3' | -54.9 | NC_004688.1 | + | 9526 | 0.66 | 0.900585 |
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Target: 5'- --aGGUCGCcaucGUACgGGUGaCCCuGCGGAg -3' miRNA: 3'- cagCCAGUGu---UAUG-CCAC-GGG-CGUCU- -5' |
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| 20606 | 3' | -54.9 | NC_004688.1 | + | 100213 | 0.66 | 0.893889 |
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Target: 5'- -gCGGUCAgCGcgGCGuUGUCCGCGGc -3' miRNA: 3'- caGCCAGU-GUuaUGCcACGGGCGUCu -5' |
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| 20606 | 3' | -54.9 | NC_004688.1 | + | 84089 | 0.66 | 0.872363 |
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Target: 5'- uUCGGUUA----GCGGggcugGCCCGCGGc -3' miRNA: 3'- cAGCCAGUguuaUGCCa----CGGGCGUCu -5' |
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| 20606 | 3' | -54.9 | NC_004688.1 | + | 33092 | 0.66 | 0.871609 |
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Target: 5'- -cCGGUCGgagugugggcCGAUGCGGUGCUUgucaaaaGCGGAc -3' miRNA: 3'- caGCCAGU----------GUUAUGCCACGGG-------CGUCU- -5' |
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| 20606 | 3' | -54.9 | NC_004688.1 | + | 49977 | 0.68 | 0.777364 |
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Target: 5'- cUUGGggGCGGUGCGGUagucgaucGCCaCGCAGAu -3' miRNA: 3'- cAGCCagUGUUAUGCCA--------CGG-GCGUCU- -5' |
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| 20606 | 3' | -54.9 | NC_004688.1 | + | 26780 | 0.69 | 0.767718 |
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Target: 5'- --gGcGUCGC--UGCGGUGgCCGCGGAg -3' miRNA: 3'- cagC-CAGUGuuAUGCCACgGGCGUCU- -5' |
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| 20606 | 3' | -54.9 | NC_004688.1 | + | 37267 | 0.69 | 0.717767 |
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Target: 5'- -gUGGgauCGAUGCGGggccggGCCCGCGGGu -3' miRNA: 3'- caGCCaguGUUAUGCCa-----CGGGCGUCU- -5' |
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| 20606 | 3' | -54.9 | NC_004688.1 | + | 69176 | 0.78 | 0.280499 |
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Target: 5'- cGUUGGUCACGAggcccuUGGaGCCCGCGGAc -3' miRNA: 3'- -CAGCCAGUGUUau----GCCaCGGGCGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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