Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2061 | 3' | -55 | NC_001348.1 | + | 122155 | 0.66 | 0.919285 |
Target: 5'- aCGaGGCCUCAacGGAACC-CGUG-CCGc -3' miRNA: 3'- gGCaCCGGGGU--UCUUGGuGUACaGGU- -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 107637 | 0.66 | 0.919285 |
Target: 5'- aCGaGGCCUCAacGGAACC-CGUG-CCGc -3' miRNA: 3'- gGCaCCGGGGU--UCUUGGuGUACaGGU- -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 84873 | 0.66 | 0.900953 |
Target: 5'- aCGUccacacaGCCUCAGGAGCCGC--GUCCGg -3' miRNA: 3'- gGCAc------CGGGGUUCUUGGUGuaCAGGU- -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 106154 | 0.66 | 0.900953 |
Target: 5'- cCUG-GGCCaCCucucGAGGGCCACcgcGUCCAa -3' miRNA: 3'- -GGCaCCGG-GG----UUCUUGGUGua-CAGGU- -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 123638 | 0.66 | 0.900953 |
Target: 5'- cCUG-GGCCaCCucucGAGGGCCACcgcGUCCAa -3' miRNA: 3'- -GGCaCCGG-GG----UUCUUGGUGua-CAGGU- -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 8123 | 0.66 | 0.894358 |
Target: 5'- uCCGUGGUCgUCAGGAGCUaacaucauuAUAUGcCCAu -3' miRNA: 3'- -GGCACCGG-GGUUCUUGG---------UGUACaGGU- -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 57130 | 0.67 | 0.886133 |
Target: 5'- aCCGccaacaGGCUCCAcaaucguacgauuAGAACCaacucggACAUGUCCGg -3' miRNA: 3'- -GGCa-----CCGGGGU-------------UCUUGG-------UGUACAGGU- -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 124423 | 0.67 | 0.880463 |
Target: 5'- uCgGUGGCCCCGGGuguGCUGCGaGaCCGc -3' miRNA: 3'- -GgCACCGGGGUUCu--UGGUGUaCaGGU- -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 105369 | 0.67 | 0.880463 |
Target: 5'- uCgGUGGCCCCGGGuguGCUGCGaGaCCGc -3' miRNA: 3'- -GgCACCGGGGUUCu--UGGUGUaCaGGU- -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 29162 | 0.67 | 0.873171 |
Target: 5'- gUGUGGgaaguCCCCGAaAAUCGCcgGUCCAu -3' miRNA: 3'- gGCACC-----GGGGUUcUUGGUGuaCAGGU- -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 42638 | 0.67 | 0.865656 |
Target: 5'- cCCGcGG-CCCAAGGcguGCCACA-GUCUAc -3' miRNA: 3'- -GGCaCCgGGGUUCU---UGGUGUaCAGGU- -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 71367 | 0.67 | 0.857924 |
Target: 5'- cCCuUGGCCCaauaAAGGAUacCGUGUCCAc -3' miRNA: 3'- -GGcACCGGGg---UUCUUGguGUACAGGU- -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 70053 | 0.68 | 0.807356 |
Target: 5'- aCCG-GGuccgaCCCCGcGAACaACAUGUCCAa -3' miRNA: 3'- -GGCaCC-----GGGGUuCUUGgUGUACAGGU- -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 123758 | 0.73 | 0.545982 |
Target: 5'- cCCGcgcgGGCCCCAAGAcCCGC--GUCCc -3' miRNA: 3'- -GGCa---CCGGGGUUCUuGGUGuaCAGGu -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 106033 | 0.73 | 0.545982 |
Target: 5'- cCCGcgcgGGCCCCAAGAcCCGC--GUCCc -3' miRNA: 3'- -GGCa---CCGGGGUUCUuGGUGuaCAGGu -5' |
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2061 | 3' | -55 | NC_001348.1 | + | 59192 | 1.11 | 0.002266 |
Target: 5'- cCCGUGGCCCCAAGAACCACAUGUCCAa -3' miRNA: 3'- -GGCACCGGGGUUCUUGGUGUACAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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