Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2061 | 5' | -52.8 | NC_001348.1 | + | 336 | 0.66 | 0.953752 |
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Target: 5'- gCGgGGUCCCAGGUCggcgGGAGuaguGCa -3' miRNA: 3'- -GCgCCAGGGUUCAGgga-CCUUuu--UGa -5' |
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| 2061 | 5' | -52.8 | NC_001348.1 | + | 82518 | 0.67 | 0.924692 |
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Target: 5'- aCGCGG-CaaGGGUCUCUGGGAAAcGCa -3' miRNA: 3'- -GCGCCaGggUUCAGGGACCUUUU-UGa -5' |
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| 2061 | 5' | -52.8 | NC_001348.1 | + | 22599 | 0.68 | 0.912969 |
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Target: 5'- --gGGUUCCugcaGGGUCCaCUGGAGGAGCc -3' miRNA: 3'- gcgCCAGGG----UUCAGG-GACCUUUUUGa -5' |
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| 2061 | 5' | -52.8 | NC_001348.1 | + | 121899 | 0.7 | 0.822429 |
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Target: 5'- aGCGGUCCCGA-UCCgaGGAuucguAAGACc -3' miRNA: 3'- gCGCCAGGGUUcAGGgaCCU-----UUUUGa -5' |
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| 2061 | 5' | -52.8 | NC_001348.1 | + | 107893 | 0.7 | 0.822429 |
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Target: 5'- aGCGGUCCCGA-UCCgaGGAuucguAAGACc -3' miRNA: 3'- gCGCCAGGGUUcAGGgaCCU-----UUUUGa -5' |
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| 2061 | 5' | -52.8 | NC_001348.1 | + | 59155 | 1.09 | 0.004719 |
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Target: 5'- cCGCGGUCCCAAGUCCCUGGAAAAACUg -3' miRNA: 3'- -GCGCCAGGGUUCAGGGACCUUUUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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