miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20614 3' -53 NC_004688.1 + 59285 0.66 0.954785
Target:  5'- ---cUCGACGauuucgaGGCCCACGUCGccGcCg -3'
miRNA:   3'- uggaAGCUGU-------UCGGGUGCAGCuuCaG- -5'
20614 3' -53 NC_004688.1 + 97886 0.66 0.94525
Target:  5'- gAUC-UCGGCGuucggguaauacucGGCCCACGUuCGGAGg- -3'
miRNA:   3'- -UGGaAGCUGU--------------UCGGGUGCA-GCUUCag -5'
20614 3' -53 NC_004688.1 + 47545 0.66 0.941959
Target:  5'- cGCCc-CGGC--GCCaCGCGUCGAGGUa -3'
miRNA:   3'- -UGGaaGCUGuuCGG-GUGCAGCUUCAg -5'
20614 3' -53 NC_004688.1 + 37080 0.66 0.931863
Target:  5'- cGCCUUCGAuCAuuuGCCCuuCGgggaucUCGAAGUa -3'
miRNA:   3'- -UGGAAGCU-GUu--CGGGu-GC------AGCUUCAg -5'
20614 3' -53 NC_004688.1 + 75844 0.67 0.926426
Target:  5'- gACUcggCGACAGGCCCAuCG-CGuuGUCc -3'
miRNA:   3'- -UGGaa-GCUGUUCGGGU-GCaGCuuCAG- -5'
20614 3' -53 NC_004688.1 + 97340 0.67 0.90856
Target:  5'- cACCUcCgGAUAGGCCUGCG-CGAcGUCg -3'
miRNA:   3'- -UGGAaG-CUGUUCGGGUGCaGCUuCAG- -5'
20614 3' -53 NC_004688.1 + 65677 0.68 0.895368
Target:  5'- cUCUUUGAUGccGGCCacgaguuCGUCGAGGUCg -3'
miRNA:   3'- uGGAAGCUGU--UCGGgu-----GCAGCUUCAG- -5'
20614 3' -53 NC_004688.1 + 34790 0.69 0.858106
Target:  5'- gGCCcgUCGAUAucuaCCACGUCGAAGcCc -3'
miRNA:   3'- -UGGa-AGCUGUucg-GGUGCAGCUUCaG- -5'
20614 3' -53 NC_004688.1 + 64013 0.7 0.787419
Target:  5'- cGCCUUCGACGaggcgcacGGCCCGCaGUucucgcacgCGggGUa -3'
miRNA:   3'- -UGGAAGCUGU--------UCGGGUG-CA---------GCuuCAg -5'
20614 3' -53 NC_004688.1 + 68674 0.72 0.711251
Target:  5'- aACCUUCGACAGGUCCGgcuUGcCGAugcgcucccaauaccAGUCg -3'
miRNA:   3'- -UGGAAGCUGUUCGGGU---GCaGCU---------------UCAG- -5'
20614 3' -53 NC_004688.1 + 36966 0.72 0.674478
Target:  5'- cGCCgUCGGCGuugaaugggguccAGCCCcccgaggggGCGUUGAGGUCg -3'
miRNA:   3'- -UGGaAGCUGU-------------UCGGG---------UGCAGCUUCAG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.