Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20614 | 5' | -49.6 | NC_004688.1 | + | 90463 | 0.66 | 0.990601 |
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Target: 5'- gGCGc---CGAGGUGGuAGAAG-CCGAg -3' miRNA: 3'- -UGCuuauGCUCCACC-UCUUCaGGUUg -5' |
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| 20614 | 5' | -49.6 | NC_004688.1 | + | 71166 | 0.67 | 0.982092 |
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Target: 5'- ---uGUugGGGGUGG-GccGUCCGGCg -3' miRNA: 3'- ugcuUAugCUCCACCuCuuCAGGUUG- -5' |
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| 20614 | 5' | -49.6 | NC_004688.1 | + | 6424 | 0.67 | 0.982092 |
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Target: 5'- gACGAGUaccgauuccgugACGcccucaagaAGGUGGA--GGUCCAGCg -3' miRNA: 3'- -UGCUUA------------UGC---------UCCACCUcuUCAGGUUG- -5' |
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| 20614 | 5' | -49.6 | NC_004688.1 | + | 71085 | 0.68 | 0.97476 |
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Target: 5'- uGCGAcUGCGGGG-GuGAGGAGUgCGAg -3' miRNA: 3'- -UGCUuAUGCUCCaC-CUCUUCAgGUUg -5' |
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| 20614 | 5' | -49.6 | NC_004688.1 | + | 28548 | 0.68 | 0.968789 |
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Target: 5'- cCGuau-CGAGGUGGAGGuuGUgCAGCg -3' miRNA: 3'- uGCuuauGCUCCACCUCUu-CAgGUUG- -5' |
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| 20614 | 5' | -49.6 | NC_004688.1 | + | 79978 | 0.69 | 0.958046 |
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Target: 5'- cCGggUugGGGGUGGgcagcGGAucGUCCGGg -3' miRNA: 3'- uGCuuAugCUCCACC-----UCUu-CAGGUUg -5' |
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| 20614 | 5' | -49.6 | NC_004688.1 | + | 79515 | 0.69 | 0.949619 |
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Target: 5'- gACGAcguUugGGGGUGGGGucaccCCAGCg -3' miRNA: 3'- -UGCUu--AugCUCCACCUCuuca-GGUUG- -5' |
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| 20614 | 5' | -49.6 | NC_004688.1 | + | 14498 | 0.7 | 0.917916 |
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Target: 5'- uCGGAgcCGucuucgcugauGGUGGAGAGGUCCAGa -3' miRNA: 3'- uGCUUauGCu----------CCACCUCUUCAGGUUg -5' |
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| 20614 | 5' | -49.6 | NC_004688.1 | + | 67928 | 0.71 | 0.891363 |
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Target: 5'- cCGGGgugGCGccGGGUGGGGAccguucuGUCCAGCg -3' miRNA: 3'- uGCUUa--UGC--UCCACCUCUu------CAGGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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