Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20615 | 3' | -47.2 | NC_004688.1 | + | 25302 | 0.66 | 0.9994 |
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Target: 5'- ----aUGAUUccGCCCAUAgaGGCGGCc- -3' miRNA: 3'- gauuaACUAGa-UGGGUAU--UCGCCGcc -5' |
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| 20615 | 3' | -47.2 | NC_004688.1 | + | 81520 | 0.67 | 0.998306 |
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Target: 5'- -----cGAUCgccACCCcgc-GCGGCGGg -3' miRNA: 3'- gauuaaCUAGa--UGGGuauuCGCCGCC- -5' |
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| 20615 | 3' | -47.2 | NC_004688.1 | + | 5010 | 0.67 | 0.997953 |
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Target: 5'- -----gGAUCUACCaGUGAGCGuGCa- -3' miRNA: 3'- gauuaaCUAGAUGGgUAUUCGC-CGcc -5' |
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| 20615 | 3' | -47.2 | NC_004688.1 | + | 97424 | 0.68 | 0.995134 |
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Target: 5'- ---cUUGAUC-ACCCAUGgaucGGUgagGGCGGg -3' miRNA: 3'- gauuAACUAGaUGGGUAU----UCG---CCGCC- -5' |
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| 20615 | 3' | -47.2 | NC_004688.1 | + | 56565 | 0.69 | 0.993357 |
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Target: 5'- ----gUGAUgUGCCCGc-GGCGGcCGGg -3' miRNA: 3'- gauuaACUAgAUGGGUauUCGCC-GCC- -5' |
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| 20615 | 3' | -47.2 | NC_004688.1 | + | 49916 | 0.7 | 0.980556 |
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Target: 5'- --uGUUGGcccUC-ACCCAguggaAAGCGGCGGa -3' miRNA: 3'- gauUAACU---AGaUGGGUa----UUCGCCGCC- -5' |
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| 20615 | 3' | -47.2 | NC_004688.1 | + | 100936 | 0.72 | 0.958869 |
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Target: 5'- aCUAAUUGAUCcGCCCAgu-GCcagGGCGu -3' miRNA: 3'- -GAUUAACUAGaUGGGUauuCG---CCGCc -5' |
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| 20615 | 3' | -47.2 | NC_004688.1 | + | 60490 | 0.74 | 0.891701 |
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Target: 5'- ----aUGGUUUACCgCGUguGAGUGGCGGg -3' miRNA: 3'- gauuaACUAGAUGG-GUA--UUCGCCGCC- -5' |
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| 20615 | 3' | -47.2 | NC_004688.1 | + | 6681 | 0.74 | 0.891701 |
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Target: 5'- --cAUUGGUCUGCCCAUGucgacgcugaugGGUGGCu- -3' miRNA: 3'- gauUAACUAGAUGGGUAU------------UCGCCGcc -5' |
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| 20615 | 3' | -47.2 | NC_004688.1 | + | 89649 | 0.84 | 0.426463 |
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Target: 5'- --uGUUGggCUguuaaaGCCCAUAAGCGGCGGa -3' miRNA: 3'- gauUAACuaGA------UGGGUAUUCGCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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