Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20619 | 5' | -62.4 | NC_004688.1 | + | 22265 | 0.66 | 0.546346 |
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Target: 5'- -cCUUUCCCuAUCuUCGCUAauCCGGGCUa -3' miRNA: 3'- aaGGAGGGGcUAG-AGCGGU--GGCCCGA- -5' |
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| 20619 | 5' | -62.4 | NC_004688.1 | + | 37044 | 0.66 | 0.536534 |
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Target: 5'- --aUUCCCgGAUgUUGgCGCCGGGCc -3' miRNA: 3'- aagGAGGGgCUAgAGCgGUGGCCCGa -5' |
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| 20619 | 5' | -62.4 | NC_004688.1 | + | 81905 | 0.66 | 0.526784 |
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Target: 5'- -gCUUUCUCGAUCUCcgacaCCAuCCGGGCg -3' miRNA: 3'- aaGGAGGGGCUAGAGc----GGU-GGCCCGa -5' |
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| 20619 | 5' | -62.4 | NC_004688.1 | + | 33893 | 0.66 | 0.520966 |
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Target: 5'- gUCCUCgggugccgcaccccaUCUGAagcgCUCGCCAUCGGGUc -3' miRNA: 3'- aAGGAG---------------GGGCUa---GAGCGGUGGCCCGa -5' |
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| 20619 | 5' | -62.4 | NC_004688.1 | + | 24114 | 0.67 | 0.45702 |
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Target: 5'- cUUCUCCaggaucgcgucaaCGAUCUCGUCAgucgucugccCCGGGCUu -3' miRNA: 3'- aAGGAGGg------------GCUAGAGCGGU----------GGCCCGA- -5' |
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| 20619 | 5' | -62.4 | NC_004688.1 | + | 33316 | 0.68 | 0.390862 |
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Target: 5'- aUCCUCUUCGGucaUCUCGUCACCccacGGGUc -3' miRNA: 3'- aAGGAGGGGCU---AGAGCGGUGG----CCCGa -5' |
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| 20619 | 5' | -62.4 | NC_004688.1 | + | 88448 | 0.7 | 0.3139 |
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Target: 5'- uUUCCUCCCUGGUCU-GCUuaUGGGCg -3' miRNA: 3'- -AAGGAGGGGCUAGAgCGGugGCCCGa -5' |
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| 20619 | 5' | -62.4 | NC_004688.1 | + | 24933 | 0.72 | 0.217704 |
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Target: 5'- aUUCCUCCCCGGUCUCGaauugaCCgcugcgaucuccaacACCGGaGCg -3' miRNA: 3'- -AAGGAGGGGCUAGAGC------GG---------------UGGCC-CGa -5' |
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| 20619 | 5' | -62.4 | NC_004688.1 | + | 95003 | 0.75 | 0.156309 |
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Target: 5'- aUCCUCgCCCGccgcaucaaaCUUGCCGCCGGGCc -3' miRNA: 3'- aAGGAG-GGGCua--------GAGCGGUGGCCCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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