Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2062 | 3' | -48.4 | NC_001348.1 | + | 60973 | 0.66 | 0.997602 |
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Target: 5'- gGUGUggAgaUCCAUAUGGCCCGAcgauauacuGUCc -3' miRNA: 3'- -UAUAaaUgaAGGUGUGCUGGGCU---------CAG- -5' |
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| 2062 | 3' | -48.4 | NC_001348.1 | + | 104034 | 0.67 | 0.995972 |
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Target: 5'- ----aUACaaCCAC-CGACCCGAGa- -3' miRNA: 3'- uauaaAUGaaGGUGuGCUGGGCUCag -5' |
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| 2062 | 3' | -48.4 | NC_001348.1 | + | 118215 | 0.67 | 0.992492 |
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Target: 5'- ---------aCCACACGGCCCGuGUg -3' miRNA: 3'- uauaaaugaaGGUGUGCUGGGCuCAg -5' |
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| 2062 | 3' | -48.4 | NC_001348.1 | + | 25462 | 0.67 | 0.992492 |
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Target: 5'- aAUAUUUACacgUUUCAUuaacuuuagGCGACCgCGGGUCa -3' miRNA: 3'- -UAUAAAUG---AAGGUG---------UGCUGG-GCUCAG- -5' |
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| 2062 | 3' | -48.4 | NC_001348.1 | + | 111577 | 0.67 | 0.992492 |
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Target: 5'- ---------aCCACACGGCCCGuGUg -3' miRNA: 3'- uauaaaugaaGGUGUGCUGGGCuCAg -5' |
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| 2062 | 3' | -48.4 | NC_001348.1 | + | 82523 | 0.68 | 0.988567 |
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Target: 5'- -------gUUCCACGCGGCaaGGGUCu -3' miRNA: 3'- uauaaaugAAGGUGUGCUGggCUCAG- -5' |
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| 2062 | 3' | -48.4 | NC_001348.1 | + | 33795 | 0.68 | 0.986955 |
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Target: 5'- -gAUUUGCgUCUugGCGuACCCGGGa- -3' miRNA: 3'- uaUAAAUGaAGGugUGC-UGGGCUCag -5' |
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| 2062 | 3' | -48.4 | NC_001348.1 | + | 66728 | 0.75 | 0.829076 |
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Target: 5'- ----gUGCUUCuCACaaGCGACUCGGGUCg -3' miRNA: 3'- uauaaAUGAAG-GUG--UGCUGGGCUCAG- -5' |
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| 2062 | 3' | -48.4 | NC_001348.1 | + | 66893 | 1.09 | 0.011118 |
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Target: 5'- aAUAUUUACUUCCACACGACCCGAGUCg -3' miRNA: 3'- -UAUAAAUGAAGGUGUGCUGGGCUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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