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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
2063 | 3' | -51.6 | NC_001348.1 | + | 107119 | 0.66 | 0.976549 |
Target: 5'- gCCCGUUgCCUCGGgGUAUgccaugCUGGCaaaGGc -3' miRNA: 3'- -GGGUAA-GGAGCUgCGUAa-----GAUCGg--UC- -5' |
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2063 | 3' | -51.6 | NC_001348.1 | + | 122673 | 0.66 | 0.976549 |
Target: 5'- gCCCGUUgCCUCGGgGUAUgccaugCUGGCaaaGGc -3' miRNA: 3'- -GGGUAA-GGAGCUgCGUAa-----GAUCGg--UC- -5' |
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2063 | 3' | -51.6 | NC_001348.1 | + | 15929 | 0.67 | 0.973899 |
Target: 5'- gCCCAUUCCcucCGugGac--CUAGCCGa -3' miRNA: 3'- -GGGUAAGGa--GCugCguaaGAUCGGUc -5' |
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2063 | 3' | -51.6 | NC_001348.1 | + | 39532 | 0.69 | 0.9121 |
Target: 5'- aCCAaUCC-CGAUGCAUUCgcGCgCGGg -3' miRNA: 3'- gGGUaAGGaGCUGCGUAAGauCG-GUC- -5' |
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2063 | 3' | -51.6 | NC_001348.1 | + | 91884 | 1.12 | 0.003941 |
Target: 5'- cCCCAUUCCUCGACGCAUUCUAGCCAGg -3' miRNA: 3'- -GGGUAAGGAGCUGCGUAAGAUCGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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