miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2063 3' -51.6 NC_001348.1 + 107119 0.66 0.976549
Target:  5'- gCCCGUUgCCUCGGgGUAUgccaugCUGGCaaaGGc -3'
miRNA:   3'- -GGGUAA-GGAGCUgCGUAa-----GAUCGg--UC- -5'
2063 3' -51.6 NC_001348.1 + 122673 0.66 0.976549
Target:  5'- gCCCGUUgCCUCGGgGUAUgccaugCUGGCaaaGGc -3'
miRNA:   3'- -GGGUAA-GGAGCUgCGUAa-----GAUCGg--UC- -5'
2063 3' -51.6 NC_001348.1 + 15929 0.67 0.973899
Target:  5'- gCCCAUUCCcucCGugGac--CUAGCCGa -3'
miRNA:   3'- -GGGUAAGGa--GCugCguaaGAUCGGUc -5'
2063 3' -51.6 NC_001348.1 + 39532 0.69 0.9121
Target:  5'- aCCAaUCC-CGAUGCAUUCgcGCgCGGg -3'
miRNA:   3'- gGGUaAGGaGCUGCGUAAGauCG-GUC- -5'
2063 3' -51.6 NC_001348.1 + 91884 1.12 0.003941
Target:  5'- cCCCAUUCCUCGACGCAUUCUAGCCAGg -3'
miRNA:   3'- -GGGUAAGGAGCUGCGUAAGAUCGGUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.