Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2063 | 5' | -44.7 | NC_001348.1 | + | 118123 | 0.66 | 0.999984 |
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Target: 5'- cGCCU--UCggacGGAUGUgcGGGGAGCu -3' miRNA: 3'- -CGGAuuAGau--UUUACGuaCCCCUUGu -5' |
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| 2063 | 5' | -44.7 | NC_001348.1 | + | 111669 | 0.66 | 0.999984 |
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Target: 5'- cGCCU--UCggacGGAUGUgcGGGGAGCu -3' miRNA: 3'- -CGGAuuAGau--UUUACGuaCCCCUUGu -5' |
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| 2063 | 5' | -44.7 | NC_001348.1 | + | 91732 | 0.66 | 0.999978 |
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Target: 5'- uCCUGG-CUAGAAUGCGucgaggaaUGGGGuGCc -3' miRNA: 3'- cGGAUUaGAUUUUACGU--------ACCCCuUGu -5' |
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| 2063 | 5' | -44.7 | NC_001348.1 | + | 44813 | 0.66 | 0.999973 |
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Target: 5'- gGCCUcAuaagugacguuguuuUCUAu--UGCAcGGGGAACAu -3' miRNA: 3'- -CGGAuU---------------AGAUuuuACGUaCCCCUUGU- -5' |
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| 2063 | 5' | -44.7 | NC_001348.1 | + | 98011 | 0.67 | 0.999944 |
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Target: 5'- aGCUuuUGAUCggcaauGUGCggGGGGGGCu -3' miRNA: 3'- -CGG--AUUAGauuu--UACGuaCCCCUUGu -5' |
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| 2063 | 5' | -44.7 | NC_001348.1 | + | 65562 | 0.67 | 0.999924 |
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Target: 5'- uGCCg---UUAugg-GCAUGGGGAAUg -3' miRNA: 3'- -CGGauuaGAUuuuaCGUACCCCUUGu -5' |
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| 2063 | 5' | -44.7 | NC_001348.1 | + | 102263 | 0.67 | 0.999924 |
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Target: 5'- aUUUAAaCUGAAAUGCGUGGGuuuGGCAa -3' miRNA: 3'- cGGAUUaGAUUUUACGUACCCc--UUGU- -5' |
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| 2063 | 5' | -44.7 | NC_001348.1 | + | 63734 | 0.68 | 0.999866 |
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Target: 5'- aGCC--GUCcaguAGUGUAUGGGGAAg- -3' miRNA: 3'- -CGGauUAGauu-UUACGUACCCCUUgu -5' |
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| 2063 | 5' | -44.7 | NC_001348.1 | + | 79550 | 0.69 | 0.999395 |
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Target: 5'- cGCCUGGUUUAAAGgcggGUAaGGGGGu-- -3' miRNA: 3'- -CGGAUUAGAUUUUa---CGUaCCCCUugu -5' |
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| 2063 | 5' | -44.7 | NC_001348.1 | + | 91849 | 1.13 | 0.017415 |
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Target: 5'- uGCCUAAUCUAAAAUGCAUGGGGAACAu -3' miRNA: 3'- -CGGAUUAGAUUUUACGUACCCCUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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