Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20633 | 3' | -60.1 | NC_004688.1 | + | 98213 | 0.66 | 0.659342 |
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Target: 5'- aCCUCGGAcuuCGGCAccagcaUGGGcagcgUGUCCACc -3' miRNA: 3'- aGGAGCCU---GCCGUac----ACCC-----ACGGGUG- -5' |
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| 20633 | 3' | -60.1 | NC_004688.1 | + | 33873 | 0.66 | 0.64916 |
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Target: 5'- gUCaUCGGgaaGGCGUGUggguccucGGGUGCCgCACc -3' miRNA: 3'- -AGgAGCCug-CCGUACA--------CCCACGG-GUG- -5' |
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| 20633 | 3' | -60.1 | NC_004688.1 | + | 27694 | 0.66 | 0.618565 |
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Target: 5'- aUCCgCGGGCGGUuggggGUGUugcgcGGGUGaugcaCCCACu -3' miRNA: 3'- -AGGaGCCUGCCG-----UACA-----CCCAC-----GGGUG- -5' |
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| 20633 | 3' | -60.1 | NC_004688.1 | + | 15900 | 0.67 | 0.59719 |
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Target: 5'- cUCCUCGGccGgGGCAuaaagaaUGUGGGUaCCCuCg -3' miRNA: 3'- -AGGAGCC--UgCCGU-------ACACCCAcGGGuG- -5' |
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| 20633 | 3' | -60.1 | NC_004688.1 | + | 20891 | 0.67 | 0.592115 |
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Target: 5'- aCCUCGGAuggaaccguggagaaCGGCAccuuuccGGUGCCUACg -3' miRNA: 3'- aGGAGCCU---------------GCCGUacac---CCACGGGUG- -5' |
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| 20633 | 3' | -60.1 | NC_004688.1 | + | 81462 | 0.67 | 0.55784 |
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Target: 5'- gCCUgCaGGCGGaAUGccUGGGUGCCCAa -3' miRNA: 3'- aGGA-GcCUGCCgUAC--ACCCACGGGUg -5' |
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| 20633 | 3' | -60.1 | NC_004688.1 | + | 102095 | 0.68 | 0.528077 |
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Target: 5'- -aCUUGGuGCGGgcCAgcgGGGUGCCCGCg -3' miRNA: 3'- agGAGCC-UGCC--GUacaCCCACGGGUG- -5' |
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| 20633 | 3' | -60.1 | NC_004688.1 | + | 80398 | 0.76 | 0.166864 |
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Target: 5'- aCCUCGGaACGGCcgucGcGGGUGUCCACg -3' miRNA: 3'- aGGAGCC-UGCCGua--CaCCCACGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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