Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2064 | 3' | -52.2 | NC_001348.1 | + | 76865 | 0.66 | 0.964603 |
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Target: 5'- gUUCCCGUGGUACcuuuACCcGuGUAAGuuuuUGUGu -3' miRNA: 3'- -AAGGGCACCAUG----UGGuC-CAUUU----ACGC- -5' |
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| 2064 | 3' | -52.2 | NC_001348.1 | + | 105899 | 0.67 | 0.953151 |
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Target: 5'- -gCCCGcgcgGGUACACaC-GGUGuAUGCGa -3' miRNA: 3'- aaGGGCa---CCAUGUG-GuCCAUuUACGC- -5' |
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| 2064 | 3' | -52.2 | NC_001348.1 | + | 124430 | 0.67 | 0.948835 |
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Target: 5'- -cCCCG-GGUGugcugcgagacCGCCGGGUGGGuuUGCGc -3' miRNA: 3'- aaGGGCaCCAU-----------GUGGUCCAUUU--ACGC- -5' |
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| 2064 | 3' | -52.2 | NC_001348.1 | + | 105362 | 0.67 | 0.948835 |
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Target: 5'- -cCCCG-GGUGugcugcgagacCGCCGGGUGGGuuUGCGc -3' miRNA: 3'- aaGGGCaCCAU-----------GUGGUCCAUUU--ACGC- -5' |
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| 2064 | 3' | -52.2 | NC_001348.1 | + | 27052 | 0.67 | 0.934343 |
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Target: 5'- aUCUCGcagGGUACACCAuGGUAAAUc-- -3' miRNA: 3'- aAGGGCa--CCAUGUGGU-CCAUUUAcgc -5' |
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| 2064 | 3' | -52.2 | NC_001348.1 | + | 123892 | 0.67 | 0.934343 |
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Target: 5'- -gCCCGccgGGUACACaC-GGUGuAUGCGa -3' miRNA: 3'- aaGGGCa--CCAUGUG-GuCCAUuUACGC- -5' |
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| 2064 | 3' | -52.2 | NC_001348.1 | + | 93910 | 0.68 | 0.928989 |
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Target: 5'- -cCCUGagagguuacgGGUACACaAGGUAAAUGCa -3' miRNA: 3'- aaGGGCa---------CCAUGUGgUCCAUUUACGc -5' |
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| 2064 | 3' | -52.2 | NC_001348.1 | + | 3679 | 0.69 | 0.869125 |
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Target: 5'- cUCCCGUGGUcAUugCAGGag---GCGu -3' miRNA: 3'- aAGGGCACCA-UGugGUCCauuuaCGC- -5' |
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| 2064 | 3' | -52.2 | NC_001348.1 | + | 67409 | 0.77 | 0.468575 |
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Target: 5'- uUUCCCGUGGUcaAgACCAuGUGAAUGCa -3' miRNA: 3'- -AAGGGCACCA--UgUGGUcCAUUUACGc -5' |
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| 2064 | 3' | -52.2 | NC_001348.1 | + | 92251 | 1.06 | 0.007219 |
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Target: 5'- cUUCCCGUGGUACACCAGGUAAAUGCGu -3' miRNA: 3'- -AAGGGCACCAUGUGGUCCAUUUACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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