Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2064 | 5' | -58.9 | NC_001348.1 | + | 120972 | 0.66 | 0.766738 |
Target: 5'- cCACACCCgauuCUCGGAG-GCCGGc- -3' miRNA: 3'- -GUGUGGGagcuGGGCCUCaUGGCCaa -5' |
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2064 | 5' | -58.9 | NC_001348.1 | + | 108820 | 0.66 | 0.766738 |
Target: 5'- cCACACCCgauuCUCGGAG-GCCGGc- -3' miRNA: 3'- -GUGUGGGagcuGGGCCUCaUGGCCaa -5' |
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2064 | 5' | -58.9 | NC_001348.1 | + | 105637 | 0.66 | 0.757294 |
Target: 5'- aACACCCUCG-CCCaGGugGGUacgucGCCGGc- -3' miRNA: 3'- gUGUGGGAGCuGGG-CC--UCA-----UGGCCaa -5' |
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2064 | 5' | -58.9 | NC_001348.1 | + | 124155 | 0.66 | 0.757294 |
Target: 5'- aACACCCUCG-CCCaGGugGGUacgucGCCGGc- -3' miRNA: 3'- gUGUGGGAGCuGGG-CC--UCA-----UGGCCaa -5' |
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2064 | 5' | -58.9 | NC_001348.1 | + | 104903 | 0.66 | 0.747741 |
Target: 5'- gGCcUCCUCu-CCCGGGGUccGCCGGg- -3' miRNA: 3'- gUGuGGGAGcuGGGCCUCA--UGGCCaa -5' |
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2064 | 5' | -58.9 | NC_001348.1 | + | 16656 | 0.66 | 0.728343 |
Target: 5'- aUACACCaCagGAgCCGaGGUACCGGUg -3' miRNA: 3'- -GUGUGG-GagCUgGGCcUCAUGGCCAa -5' |
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2064 | 5' | -58.9 | NC_001348.1 | + | 17585 | 0.68 | 0.63497 |
Target: 5'- uGCGCCa-CGGCCCGGgaaaaccacauaugGGUGCUGGUc -3' miRNA: 3'- gUGUGGgaGCUGGGCC--------------UCAUGGCCAa -5' |
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2064 | 5' | -58.9 | NC_001348.1 | + | 42496 | 0.68 | 0.617685 |
Target: 5'- gCACGCCUUgGGCCgCGGgcGGUGauCCGGUUg -3' miRNA: 3'- -GUGUGGGAgCUGG-GCC--UCAU--GGCCAA- -5' |
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2064 | 5' | -58.9 | NC_001348.1 | + | 71372 | 0.69 | 0.577201 |
Target: 5'- cCACACCCUUGGCCCaauaaaGGA-UACCGuGUc -3' miRNA: 3'- -GUGUGGGAGCUGGG------CCUcAUGGC-CAa -5' |
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2064 | 5' | -58.9 | NC_001348.1 | + | 92217 | 1.05 | 0.002519 |
Target: 5'- aCACACCCUCGACCCGGAGUACCGGUUc -3' miRNA: 3'- -GUGUGGGAGCUGGGCCUCAUGGCCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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