miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20646 3' -52.1 NC_004688.1 + 46716 0.67 0.925286
Target:  5'- -gGUCGGGUcuGCGGGGCUGAa---- -3'
miRNA:   3'- uaCAGUUCAcuUGCCCCGGCUcuuuc -5'
20646 3' -52.1 NC_004688.1 + 34416 0.67 0.91945
Target:  5'- ----aAGGUGAGCGGgaacgcGGCCGAGAGc- -3'
miRNA:   3'- uacagUUCACUUGCC------CCGGCUCUUuc -5'
20646 3' -52.1 NC_004688.1 + 20715 0.68 0.886314
Target:  5'- cGUGgaUCAGGUGAGgcaucccgcuauCGGGGUCGAGGu-- -3'
miRNA:   3'- -UAC--AGUUCACUU------------GCCCCGGCUCUuuc -5'
20646 3' -52.1 NC_004688.1 + 94670 0.74 0.582486
Target:  5'- -cGUCGAGcUGGcgGCGGGGCCGugcgcagcGGGAAGg -3'
miRNA:   3'- uaCAGUUC-ACU--UGCCCCGGC--------UCUUUC- -5'
20646 3' -52.1 NC_004688.1 + 68074 0.76 0.478381
Target:  5'- --aUCAAGUGAGuggguuaGGGGCCGAGAAGu -3'
miRNA:   3'- uacAGUUCACUUg------CCCCGGCUCUUUc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.