Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2065 | 3' | -52.6 | NC_001348.1 | + | 110492 | 0.66 | 0.962329 |
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Target: 5'- aCGCG---GGGCGAcucguccgagucaaaACCCGGGGcaUCGGUu -3' miRNA: 3'- -GCGCauuUCCGCU---------------UGGGCUCU--AGCUA- -5' |
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| 2065 | 3' | -52.6 | NC_001348.1 | + | 119300 | 0.66 | 0.962329 |
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Target: 5'- aCGCG---GGGCGAcucguccgagucaaaACCCGGGGcaUCGGUu -3' miRNA: 3'- -GCGCauuUCCGCU---------------UGGGCUCU--AGCUA- -5' |
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| 2065 | 3' | -52.6 | NC_001348.1 | + | 124736 | 0.66 | 0.957116 |
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Target: 5'- gGCGccc-GGCGGACCCGGGAg---- -3' miRNA: 3'- gCGCauuuCCGCUUGGGCUCUagcua -5' |
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| 2065 | 3' | -52.6 | NC_001348.1 | + | 107226 | 0.67 | 0.939585 |
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Target: 5'- cCGCGUGGGGGaGAGCCCaGAGggCc-- -3' miRNA: 3'- -GCGCAUUUCCgCUUGGG-CUCuaGcua -5' |
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| 2065 | 3' | -52.6 | NC_001348.1 | + | 122566 | 0.67 | 0.939585 |
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Target: 5'- cCGCGUGGGGGaGAGCCCaGAGggCc-- -3' miRNA: 3'- -GCGCAUUUCCgCUUGGG-CUCuaGcua -5' |
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| 2065 | 3' | -52.6 | NC_001348.1 | + | 121480 | 0.68 | 0.930913 |
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Target: 5'- aGCGUucccgcuaagggggcAAAGGCGGACCUgccguuuGAGAcCGAUg -3' miRNA: 3'- gCGCA---------------UUUCCGCUUGGG-------CUCUaGCUA- -5' |
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| 2065 | 3' | -52.6 | NC_001348.1 | + | 108312 | 0.68 | 0.930913 |
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Target: 5'- aGCGUucccgcuaagggggcAAAGGCGGACCUgccguuuGAGAcCGAUg -3' miRNA: 3'- gCGCA---------------UUUCCGCUUGGG-------CUCUaGCUA- -5' |
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| 2065 | 3' | -52.6 | NC_001348.1 | + | 97668 | 0.7 | 0.854837 |
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Target: 5'- aGCGguuaauGGGGCGggUgCCGGGAUUGAc -3' miRNA: 3'- gCGCau----UUCCGCuuG-GGCUCUAGCUa -5' |
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| 2065 | 3' | -52.6 | NC_001348.1 | + | 100367 | 1.06 | 0.007619 |
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Target: 5'- aCGCGUAAAGGCGAACCCGAGAUCGAUu -3' miRNA: 3'- -GCGCAUUUCCGCUUGGGCUCUAGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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