miRNA: 3'- uaUAUUg--UGAGGUUAgCUGGGAGAAc -5'
miRNA: 3'- -UAUAUUGUGAGGUU----AGCUGGgAGAac -5'
miRNA: 3'- -UAUAUUGu-GAGGUUAgCUGGGAgaac -5'
miRNA: 3'- -UAUAUUGUGAGGUUAGCUgGGAGaac -5'
miRNA: 3'- -UAUAUUGUGAGGUUAGCUGGGAGAAC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2065 | 5' | -47.1 | NC_001348.1 | + | 35668 | 0.68 | 0.996926 |
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Target: 5'- -aAUAAaaaGCUCCAAUgGAUCCUUUUc -3' miRNA: 3'- uaUAUUg--UGAGGUUAgCUGGGAGAAc -5' |
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| 2065 | 5' | -47.1 | NC_001348.1 | + | 75288 | 0.71 | 0.977236 |
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Target: 5'- uAUAUAACAUUCCAGaugcUCGACC-UCUa- -3' miRNA: 3'- -UAUAUUGUGAGGUU----AGCUGGgAGAac -5' |
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| 2065 | 5' | -47.1 | NC_001348.1 | + | 22756 | 0.72 | 0.961229 |
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Target: 5'- uGUGUGGCucCUCCAGUgGACCCUg--- -3' miRNA: 3'- -UAUAUUGu-GAGGUUAgCUGGGAgaac -5' |
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| 2065 | 5' | -47.1 | NC_001348.1 | + | 100203 | 0.78 | 0.729575 |
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Target: 5'- uAUAUAACACUCCAAUCGA-UCUCg-- -3' miRNA: 3'- -UAUAUUGUGAGGUUAGCUgGGAGaac -5' |
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| 2065 | 5' | -47.1 | NC_001348.1 | + | 100332 | 1.06 | 0.022842 |
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Target: 5'- uAUAUAACACUCCAAUCGACCCUCUUGc -3' miRNA: 3'- -UAUAUUGUGAGGUUAGCUGGGAGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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