miRNA: 3'- -GUGAa---CAGaa--GGGGg--AGCCGAuGUUG- -5'
miRNA: 3'- -GUGAac---AGAAGGgGGAGCCGa----UGUUG- -5'
miRNA: 3'- -GUGAACAgaAGGgGGAGCCGau-----GUUG- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20670 | 3' | -53.2 | NC_004688.1 | + | 32112 | 0.66 | 0.930387 |
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Target: 5'- aCACUccgaGUCggugCCCCagaUCGGCUuCGGCg -3' miRNA: 3'- -GUGAa---CAGaa--GGGGg--AGCCGAuGUUG- -5' |
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| 20670 | 3' | -53.2 | NC_004688.1 | + | 25568 | 0.67 | 0.90665 |
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Target: 5'- cCGCUcaaccUCUUCCgCCUCGGCcucccGCAGCc -3' miRNA: 3'- -GUGAac---AGAAGGgGGAGCCGa----UGUUG- -5' |
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| 20670 | 3' | -53.2 | NC_004688.1 | + | 54352 | 0.68 | 0.847094 |
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Target: 5'- aCGCUUGUagUCCgCCUCGGCcucaagcCAGCg -3' miRNA: 3'- -GUGAACAgaAGGgGGAGCCGau-----GUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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