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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20674 | 5' | -52.6 | NC_004688.1 | + | 98803 | 0.66 | 0.951031 |
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Target: 5'- --gUCGCGGCGCUguaGUCGuaguGGUACa--- -3' miRNA: 3'- cgaAGCGCCGCGA---UAGC----UCAUGgauc -5' |
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| 20674 | 5' | -52.6 | NC_004688.1 | + | 3990 | 0.67 | 0.931863 |
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Target: 5'- aGCUUCGCGGCcgaaCUGUCcAGccgaACCUAa -3' miRNA: 3'- -CGAAGCGCCGc---GAUAGcUCa---UGGAUc -5' |
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| 20674 | 5' | -52.6 | NC_004688.1 | + | 49397 | 0.67 | 0.920146 |
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Target: 5'- cGCUUCGCggagcauGGUGCUGUCG-GUcaugacGCCUc- -3' miRNA: 3'- -CGAAGCG-------CCGCGAUAGCuCA------UGGAuc -5' |
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| 20674 | 5' | -52.6 | NC_004688.1 | + | 35002 | 0.69 | 0.872961 |
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Target: 5'- aGCUUCGCGG-GCUugggccuAUUGAGUuccGCCUc- -3' miRNA: 3'- -CGAAGCGCCgCGA-------UAGCUCA---UGGAuc -5' |
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| 20674 | 5' | -52.6 | NC_004688.1 | + | 100212 | 0.71 | 0.777801 |
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Target: 5'- gGCggucagCGCGGCGUUGUCcgcgGCCUGGa -3' miRNA: 3'- -CGaa----GCGCCGCGAUAGcucaUGGAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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