Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2068 | 5' | -57 | NC_001348.1 | + | 104028 | 0.66 | 0.787478 |
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Target: 5'- gAUGGacuCCcGACUCGGUccugggcuuGGUgGCAGAAGa -3' miRNA: 3'- -UACC---GGuCUGAGCCG---------CCAgUGUCUUC- -5' |
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| 2068 | 5' | -57 | NC_001348.1 | + | 97895 | 0.67 | 0.749173 |
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Target: 5'- -aGGCCguuacuuuGGACUCGGgggcuuUGGUaaaGCAGAAGg -3' miRNA: 3'- uaCCGG--------UCUGAGCC------GCCAg--UGUCUUC- -5' |
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| 2068 | 5' | -57 | NC_001348.1 | + | 95281 | 0.67 | 0.739307 |
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Target: 5'- uUGGCCAGAggagCGGCGGuugUCACAa--- -3' miRNA: 3'- uACCGGUCUga--GCCGCC---AGUGUcuuc -5' |
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| 2068 | 5' | -57 | NC_001348.1 | + | 102622 | 0.69 | 0.636911 |
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Target: 5'- aAUGGCCGGAaggUGGCGGgggUGguGAAGg -3' miRNA: 3'- -UACCGGUCUga-GCCGCCa--GUguCUUC- -5' |
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| 2068 | 5' | -57 | NC_001348.1 | + | 123588 | 1.07 | 0.002062 |
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Target: 5'- gAUGGCCAGACUCGGCGGUCACAGAAGg -3' miRNA: 3'- -UACCGGUCUGAGCCGCCAGUGUCUUC- -5' |
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| 2068 | 5' | -57 | NC_001348.1 | + | 106204 | 1.07 | 0.002062 |
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Target: 5'- gAUGGCCAGACUCGGCGGUCACAGAAGg -3' miRNA: 3'- -UACCGGUCUGAGCCGCCAGUGUCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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