Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2069 | 3' | -60.9 | NC_001348.1 | + | 124576 | 0.66 | 0.62429 |
Target: 5'- aGUCCCCuccuuuucUCGuGAGCGCCaCUgGCGcGCGg -3' miRNA: 3'- -CGGGGG--------AGCuCUCGUGG-GAgCGC-UGU- -5' |
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2069 | 3' | -60.9 | NC_001348.1 | + | 105216 | 0.66 | 0.62429 |
Target: 5'- aGUCCCCuccuuuucUCGuGAGCGCCaCUgGCGcGCGg -3' miRNA: 3'- -CGGGGG--------AGCuCUCGUGG-GAgCGC-UGU- -5' |
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2069 | 3' | -60.9 | NC_001348.1 | + | 51748 | 0.67 | 0.594366 |
Target: 5'- uGCCCCCUCGuGAuuuucaucaaaaGCAaggugggccgcUCCUCggGCGACGg -3' miRNA: 3'- -CGGGGGAGCuCU------------CGU-----------GGGAG--CGCUGU- -5' |
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2069 | 3' | -60.9 | NC_001348.1 | + | 111622 | 0.67 | 0.584436 |
Target: 5'- aGCCCCUcCGAGAG-ACCgUgCGCGAg- -3' miRNA: 3'- -CGGGGGaGCUCUCgUGGgA-GCGCUgu -5' |
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2069 | 3' | -60.9 | NC_001348.1 | + | 118170 | 0.67 | 0.584436 |
Target: 5'- aGCCCCUcCGAGAG-ACCgUgCGCGAg- -3' miRNA: 3'- -CGGGGGaGCUCUCgUGGgA-GCGCUgu -5' |
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2069 | 3' | -60.9 | NC_001348.1 | + | 17 | 0.67 | 0.567632 |
Target: 5'- gGCCCCCUCGAGAgagaaaaaaaaaaGCgACCCcaccuccccgcgcguUUGCGGg- -3' miRNA: 3'- -CGGGGGAGCUCU-------------CG-UGGG---------------AGCGCUgu -5' |
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2069 | 3' | -60.9 | NC_001348.1 | + | 105015 | 0.67 | 0.567632 |
Target: 5'- gGCCCCCUCGAGAgagaaaaaaaaaaGCgACCCcaccuccccgcgcguUUGCGGg- -3' miRNA: 3'- -CGGGGGAGCUCU-------------CG-UGGG---------------AGCGCUgu -5' |
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2069 | 3' | -60.9 | NC_001348.1 | + | 74258 | 0.67 | 0.564679 |
Target: 5'- cGCCCCCgaugCGGGAGCcGCCa---CGGCAa -3' miRNA: 3'- -CGGGGGa---GCUCUCG-UGGgagcGCUGU- -5' |
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2069 | 3' | -60.9 | NC_001348.1 | + | 107074 | 0.73 | 0.281196 |
Target: 5'- aGCCUCCUCGAcaagauuaggGAGaCACCCgUCGCgGACGc -3' miRNA: 3'- -CGGGGGAGCU----------CUC-GUGGG-AGCG-CUGU- -5' |
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2069 | 3' | -60.9 | NC_001348.1 | + | 118432 | 0.76 | 0.183275 |
Target: 5'- cCCCCCUCGcccaacgcgguGAGCGCUUUCGCGGCu -3' miRNA: 3'- cGGGGGAGCu----------CUCGUGGGAGCGCUGu -5' |
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2069 | 3' | -60.9 | NC_001348.1 | + | 111360 | 0.76 | 0.183275 |
Target: 5'- cCCCCCUCGcccaacgcgguGAGCGCUUUCGCGGCu -3' miRNA: 3'- cGGGGGAGCu----------CUCGUGGGAGCGCUGu -5' |
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2069 | 3' | -60.9 | NC_001348.1 | + | 122718 | 0.78 | 0.129244 |
Target: 5'- aGCCUCCUCGAcaagauuaggGAGaCACCCgUCGCGGCGc -3' miRNA: 3'- -CGGGGGAGCU----------CUC-GUGGG-AGCGCUGU- -5' |
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2069 | 3' | -60.9 | NC_001348.1 | + | 123215 | 1.1 | 0.000792 |
Target: 5'- gGCCCCCUCGAGAGCACCCUCGCGACAg -3' miRNA: 3'- -CGGGGGAGCUCUCGUGGGAGCGCUGU- -5' |
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2069 | 3' | -60.9 | NC_001348.1 | + | 106577 | 1.1 | 0.000792 |
Target: 5'- gGCCCCCUCGAGAGCACCCUCGCGACAg -3' miRNA: 3'- -CGGGGGAGCUCUCGUGGGAGCGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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