Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20695 | 3' | -59.9 | NC_004688.1 | + | 107739 | 0.66 | 0.683281 |
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Target: 5'- ---aUCCUCGagCGGCGGcagCGgGCCAc -3' miRNA: 3'- cuuaAGGGGCg-GCCGCCaa-GCgCGGU- -5' |
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| 20695 | 3' | -59.9 | NC_004688.1 | + | 103226 | 0.66 | 0.683281 |
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Target: 5'- ---aUCgCCGUCGGcCGGUUCGCcucGUCGc -3' miRNA: 3'- cuuaAGgGGCGGCC-GCCAAGCG---CGGU- -5' |
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| 20695 | 3' | -59.9 | NC_004688.1 | + | 76513 | 0.66 | 0.673178 |
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Target: 5'- ---cUCCCgGCUGGcCGGUcucCGCGCgGg -3' miRNA: 3'- cuuaAGGGgCGGCC-GCCAa--GCGCGgU- -5' |
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| 20695 | 3' | -59.9 | NC_004688.1 | + | 704 | 0.66 | 0.673178 |
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Target: 5'- ---cUCuCCCGCUGGac--UCGCGCCAc -3' miRNA: 3'- cuuaAG-GGGCGGCCgccaAGCGCGGU- -5' |
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| 20695 | 3' | -59.9 | NC_004688.1 | + | 46953 | 0.67 | 0.601988 |
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Target: 5'- ------aCCGCCucGGCGGggCGUGCCGu -3' miRNA: 3'- cuuaaggGGCGG--CCGCCaaGCGCGGU- -5' |
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| 20695 | 3' | -59.9 | NC_004688.1 | + | 90663 | 0.68 | 0.541705 |
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Target: 5'- uGAUUCCCgGCUGcaGCaGGUUCGCGUa- -3' miRNA: 3'- cUUAAGGGgCGGC--CG-CCAAGCGCGgu -5' |
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| 20695 | 3' | -59.9 | NC_004688.1 | + | 67526 | 0.68 | 0.531833 |
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Target: 5'- ----cCCCUGCUGGCGcaaUCGCGCUc -3' miRNA: 3'- cuuaaGGGGCGGCCGCca-AGCGCGGu -5' |
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| 20695 | 3' | -59.9 | NC_004688.1 | + | 96857 | 0.68 | 0.522028 |
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Target: 5'- ---cUUCUCGUCGGCGGUUUccauCGCCAc -3' miRNA: 3'- cuuaAGGGGCGGCCGCCAAGc---GCGGU- -5' |
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| 20695 | 3' | -59.9 | NC_004688.1 | + | 66772 | 0.74 | 0.24426 |
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Target: 5'- cAGUUCCCCGuCCGGguugaGGaugucgUCGCGCCAg -3' miRNA: 3'- cUUAAGGGGC-GGCCg----CCa-----AGCGCGGU- -5' |
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| 20695 | 3' | -59.9 | NC_004688.1 | + | 109008 | 0.74 | 0.238457 |
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Target: 5'- aGGAUggugCCagGCCcGCGGUUCGCGCCAa -3' miRNA: 3'- -CUUAa---GGggCGGcCGCCAAGCGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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