Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20696 | 5' | -52.8 | NC_004688.1 | + | 85476 | 0.66 | 0.963331 |
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Target: 5'- gCCGGUggGGUUgaUGuuGUUCGGGUu -3' miRNA: 3'- -GGCUGuuCUAAgaACggCAGGCCCAc -5' |
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| 20696 | 5' | -52.8 | NC_004688.1 | + | 2982 | 0.66 | 0.963331 |
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Target: 5'- cCCGcgagcaucagguGCAGGAUUCUgggaccGUgGUuuGGGUGa -3' miRNA: 3'- -GGC------------UGUUCUAAGAa-----CGgCAggCCCAC- -5' |
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| 20696 | 5' | -52.8 | NC_004688.1 | + | 105774 | 0.66 | 0.951832 |
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Target: 5'- gUCGACGAG-UUCgcGaCCGUCaGGGUGc -3' miRNA: 3'- -GGCUGUUCuAAGaaC-GGCAGgCCCAC- -5' |
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| 20696 | 5' | -52.8 | NC_004688.1 | + | 83312 | 0.67 | 0.942951 |
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Target: 5'- cCCGACGGGGUccagCggGCCcggCCGGGg- -3' miRNA: 3'- -GGCUGUUCUAa---GaaCGGca-GGCCCac -5' |
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| 20696 | 5' | -52.8 | NC_004688.1 | + | 86657 | 0.67 | 0.933067 |
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Target: 5'- cCCGugGGGAccgUUGCCGgugcCCGGGg- -3' miRNA: 3'- -GGCugUUCUaagAACGGCa---GGCCCac -5' |
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| 20696 | 5' | -52.8 | NC_004688.1 | + | 83529 | 0.68 | 0.897332 |
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Target: 5'- aCC-ACugGAauUUCUggaUGCCGUCgCGGGUGu -3' miRNA: 3'- -GGcUGuuCU--AAGA---ACGGCAG-GCCCAC- -5' |
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| 20696 | 5' | -52.8 | NC_004688.1 | + | 32043 | 0.68 | 0.890502 |
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Target: 5'- gCCGGCAAGGguugaCUUGCCuaGcCCGGGc- -3' miRNA: 3'- -GGCUGUUCUaa---GAACGG--CaGGCCCac -5' |
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| 20696 | 5' | -52.8 | NC_004688.1 | + | 9402 | 0.69 | 0.88343 |
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Target: 5'- gUCGugGAG-UUCgUGCCGgCCGGGg- -3' miRNA: 3'- -GGCugUUCuAAGaACGGCaGGCCCac -5' |
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| 20696 | 5' | -52.8 | NC_004688.1 | + | 85312 | 0.69 | 0.861599 |
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Target: 5'- gCGAUggGGgcguucaUCUUGCCGaucugcccaggaccgCCGGGUGa -3' miRNA: 3'- gGCUGuuCUa------AGAACGGCa--------------GGCCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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