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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2070 | 5' | -48.3 | NC_001348.1 | + | 14793 | 0.66 | 0.999177 |
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Target: 5'- uGCUGUGGgccAAgGAacgGCCAUUUGGCa -3' miRNA: 3'- gCGGCAUUa--UUgCUa--CGGUGAACUGc -5' |
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| 2070 | 5' | -48.3 | NC_001348.1 | + | 34094 | 0.74 | 0.893868 |
|
Target: 5'- aGCCGUAAUcaauuuGACGGUGCC-CUggGACc -3' miRNA: 3'- gCGGCAUUA------UUGCUACGGuGAa-CUGc -5' |
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| 2070 | 5' | -48.3 | NC_001348.1 | + | 36479 | 0.69 | 0.988045 |
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Target: 5'- aGCCGUugaAugGGUGCCguACUUG-CGg -3' miRNA: 3'- gCGGCAuuaUugCUACGG--UGAACuGC- -5' |
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| 2070 | 5' | -48.3 | NC_001348.1 | + | 38104 | 0.67 | 0.995678 |
|
Target: 5'- gGCUGUAGUuucAC-AUGCCGCUacaaccUGACGu -3' miRNA: 3'- gCGGCAUUAu--UGcUACGGUGA------ACUGC- -5' |
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| 2070 | 5' | -48.3 | NC_001348.1 | + | 50189 | 0.66 | 0.998184 |
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Target: 5'- aGCCGag--GugGAUaCCGCUUGugGc -3' miRNA: 3'- gCGGCauuaUugCUAcGGUGAACugC- -5' |
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| 2070 | 5' | -48.3 | NC_001348.1 | + | 106944 | 1.01 | 0.043067 |
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Target: 5'- cCGCCGUAAUAACGAUGCCACUUG-CGg -3' miRNA: 3'- -GCGGCAUUAUUGCUACGGUGAACuGC- -5' |
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| 2070 | 5' | -48.3 | NC_001348.1 | + | 122847 | 1.12 | 0.009993 |
|
Target: 5'- cCGCCGUAAUAACGAUGCCACUUGACGg -3' miRNA: 3'- -GCGGCAUUAUUGCUACGGUGAACUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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