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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20701 | 5' | -59.5 | NC_004688.1 | + | 17661 | 0.66 | 0.70619 |
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Target: 5'- aGggCUCGG-CGggcuuGCCACgaUGGCGGACc -3' miRNA: 3'- -CaaGAGCCaGC-----CGGUGg-ACCGCCUGu -5' |
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| 20701 | 5' | -59.5 | NC_004688.1 | + | 54611 | 0.66 | 0.665814 |
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Target: 5'- --aCUUGGU-GGUCACCUcGGcCGGAUAg -3' miRNA: 3'- caaGAGCCAgCCGGUGGA-CC-GCCUGU- -5' |
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| 20701 | 5' | -59.5 | NC_004688.1 | + | 88275 | 0.67 | 0.625 |
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Target: 5'- cUUCUUgucgaGGUCGGCCuuGCC-GGCGG-CAa -3' miRNA: 3'- cAAGAG-----CCAGCCGG--UGGaCCGCCuGU- -5' |
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| 20701 | 5' | -59.5 | NC_004688.1 | + | 62761 | 0.68 | 0.582243 |
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Target: 5'- cUUCUCGGccgcgguagaggCGGCCAacccuaaCUGGCGGgaGCAa -3' miRNA: 3'- cAAGAGCCa-----------GCCGGUg------GACCGCC--UGU- -5' |
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| 20701 | 5' | -59.5 | NC_004688.1 | + | 93190 | 0.74 | 0.245066 |
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Target: 5'- gGUUC-CGGcgCgGGCCGCUUGGCGGGCc -3' miRNA: 3'- -CAAGaGCCa-G-CCGGUGGACCGCCUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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