Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20705 | 5' | -58.7 | NC_004688.1 | + | 45412 | 0.66 | 0.710456 |
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Target: 5'- -aCGGguGGCCC-UCCGGGaUGCg-- -3' miRNA: 3'- caGUCguCUGGGuAGGCCCaACGggu -5' |
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| 20705 | 5' | -58.7 | NC_004688.1 | + | 99754 | 0.66 | 0.700318 |
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Target: 5'- gGUCAGCAcGCCCAgguuguugaacCCGGGgccGCUCGg -3' miRNA: 3'- -CAGUCGUcUGGGUa----------GGCCCaa-CGGGU- -5' |
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| 20705 | 5' | -58.7 | NC_004688.1 | + | 13979 | 0.66 | 0.690121 |
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Target: 5'- ---cGUAGACCCGagaaaacccaauUCCGGaGUUGCCg- -3' miRNA: 3'- caguCGUCUGGGU------------AGGCC-CAACGGgu -5' |
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| 20705 | 5' | -58.7 | NC_004688.1 | + | 46447 | 0.66 | 0.679875 |
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Target: 5'- -aCAGCGccguGACCCAUCCGauUUGCgCCGu -3' miRNA: 3'- caGUCGU----CUGGGUAGGCccAACG-GGU- -5' |
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| 20705 | 5' | -58.7 | NC_004688.1 | + | 83323 | 0.67 | 0.659271 |
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Target: 5'- -cCAGCGGGCCCggCCGGGgaucaggaagUUGaCCGc -3' miRNA: 3'- caGUCGUCUGGGuaGGCCC----------AACgGGU- -5' |
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| 20705 | 5' | -58.7 | NC_004688.1 | + | 65899 | 0.67 | 0.659271 |
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Target: 5'- -cCGGUGGGCUCGUaugCCGGGgcGUCCAc -3' miRNA: 3'- caGUCGUCUGGGUA---GGCCCaaCGGGU- -5' |
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| 20705 | 5' | -58.7 | NC_004688.1 | + | 60963 | 0.68 | 0.586893 |
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Target: 5'- uGUUGGCacgGGGCCUAUagCGGGuUUGCCCGg -3' miRNA: 3'- -CAGUCG---UCUGGGUAg-GCCC-AACGGGU- -5' |
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| 20705 | 5' | -58.7 | NC_004688.1 | + | 66766 | 0.68 | 0.586893 |
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Target: 5'- aUCGuGCAGuucCCCGUCCGGGUUgaggaugucgucGCgCCAg -3' miRNA: 3'- cAGU-CGUCu--GGGUAGGCCCAA------------CG-GGU- -5' |
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| 20705 | 5' | -58.7 | NC_004688.1 | + | 68924 | 0.69 | 0.526034 |
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Target: 5'- ---uGCGGGCCuUGUCCcGGUUGCCCGg -3' miRNA: 3'- caguCGUCUGG-GUAGGcCCAACGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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