Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20707 | 3' | -53.2 | NC_004688.1 | + | 4347 | 0.66 | 0.951571 |
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Target: 5'- cACUCCGgagUGaaGCGUug-UGUgGGUAGCa -3' miRNA: 3'- -UGAGGCa--GC--CGCAuaaGCAgCCAUCG- -5' |
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| 20707 | 3' | -53.2 | NC_004688.1 | + | 52951 | 0.67 | 0.921961 |
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Target: 5'- gGC-CUGUggCGGUGUAggcgaagUCGUCGGUguccAGCg -3' miRNA: 3'- -UGaGGCA--GCCGCAUa------AGCAGCCA----UCG- -5' |
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| 20707 | 3' | -53.2 | NC_004688.1 | + | 83102 | 0.68 | 0.903765 |
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Target: 5'- gACUccCCGUCGacauCGacgUCGUCGGUGGUg -3' miRNA: 3'- -UGA--GGCAGCc---GCauaAGCAGCCAUCG- -5' |
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| 20707 | 3' | -53.2 | NC_004688.1 | + | 90922 | 0.68 | 0.903765 |
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Target: 5'- aGCUuuG-CGGCcUGggCGUCGGUcAGCu -3' miRNA: 3'- -UGAggCaGCCGcAUaaGCAGCCA-UCG- -5' |
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| 20707 | 3' | -53.2 | NC_004688.1 | + | 65755 | 0.68 | 0.880469 |
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Target: 5'- aGCUCCGaUGGUuugcuugagcUCGUCGGUGGUg -3' miRNA: 3'- -UGAGGCaGCCGcaua------AGCAGCCAUCG- -5' |
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| 20707 | 3' | -53.2 | NC_004688.1 | + | 101913 | 0.7 | 0.810105 |
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Target: 5'- -gUCC-UCGGgGaacUCGUCGGUGGCu -3' miRNA: 3'- ugAGGcAGCCgCauaAGCAGCCAUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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