Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2071 | 3' | -55.2 | NC_001348.1 | + | 57537 | 0.66 | 0.872245 |
Target: 5'- cGUA-CAUGGUUgCCGGAACCCCcggaacauauaggacGGGCa -3' miRNA: 3'- -UAUcGUACCGA-GGUCUUGGGG---------------UUCGa -5' |
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2071 | 3' | -55.2 | NC_001348.1 | + | 88150 | 0.66 | 0.853237 |
Target: 5'- -aAGCAaGGCUguuguuuuaCCAGAGCCCauagGGGCa -3' miRNA: 3'- uaUCGUaCCGA---------GGUCUUGGGg---UUCGa -5' |
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2071 | 3' | -55.2 | NC_001348.1 | + | 46253 | 0.67 | 0.791018 |
Target: 5'- --uGCGUGuacGCUCCAGAACgCCAuGCc -3' miRNA: 3'- uauCGUAC---CGAGGUCUUGgGGUuCGa -5' |
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2071 | 3' | -55.2 | NC_001348.1 | + | 49331 | 0.67 | 0.781427 |
Target: 5'- -cAGCAcagucGGCUCCGGuAACCCCGcGUc -3' miRNA: 3'- uaUCGUa----CCGAGGUC-UUGGGGUuCGa -5' |
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2071 | 3' | -55.2 | NC_001348.1 | + | 90349 | 0.7 | 0.647232 |
Target: 5'- uGUAGCAUGGCUUCGGcAugCggggCCAAGUg -3' miRNA: 3'- -UAUCGUACCGAGGUC-UugG----GGUUCGa -5' |
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2071 | 3' | -55.2 | NC_001348.1 | + | 51142 | 0.71 | 0.551574 |
Target: 5'- cGUAGUgcGuGCUCCAGAaaccACCgCCAGGCUg -3' miRNA: 3'- -UAUCGuaC-CGAGGUCU----UGG-GGUUCGA- -5' |
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2071 | 3' | -55.2 | NC_001348.1 | + | 39854 | 0.73 | 0.490133 |
Target: 5'- cAUAGCAUGGUUCCAaguuuAACCCCAA-CUg -3' miRNA: 3'- -UAUCGUACCGAGGUc----UUGGGGUUcGA- -5' |
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2071 | 3' | -55.2 | NC_001348.1 | + | 122261 | 1.06 | 0.0031 |
Target: 5'- uAUAGCAUGGCUCCAGAACCCCAAGCUg -3' miRNA: 3'- -UAUCGUACCGAGGUCUUGGGGUUCGA- -5' |
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2071 | 3' | -55.2 | NC_001348.1 | + | 107531 | 1.06 | 0.0031 |
Target: 5'- uAUAGCAUGGCUCCAGAACCCCAAGCUg -3' miRNA: 3'- -UAUCGUACCGAGGUCUUGGGGUUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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