Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20712 | 3' | -54.7 | NC_004688.1 | + | 75024 | 0.66 | 0.896358 |
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Target: 5'- --gGGCGgGGCGaucCCCGACUGCu-- -3' miRNA: 3'- acaCCGCgCUGUau-GGGCUGAUGcuu -5' |
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| 20712 | 3' | -54.7 | NC_004688.1 | + | 80952 | 0.67 | 0.859469 |
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Target: 5'- aGUGGUGCGAUAUuccACCCGuCcgggGCGu- -3' miRNA: 3'- aCACCGCGCUGUA---UGGGCuGa---UGCuu -5' |
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| 20712 | 3' | -54.7 | NC_004688.1 | + | 27492 | 0.67 | 0.851404 |
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Target: 5'- --aGGCGgcUGAuCGUGCCCGGCUGCu-- -3' miRNA: 3'- acaCCGC--GCU-GUAUGGGCUGAUGcuu -5' |
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| 20712 | 3' | -54.7 | NC_004688.1 | + | 41496 | 0.67 | 0.851404 |
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Target: 5'- -aUGGCGUGACuugggcAUuuGACUGCGGu -3' miRNA: 3'- acACCGCGCUGua----UGggCUGAUGCUu -5' |
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| 20712 | 3' | -54.7 | NC_004688.1 | + | 5590 | 0.67 | 0.834641 |
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Target: 5'- gGUGGCGCG-CGacUACCCaACUGCc-- -3' miRNA: 3'- aCACCGCGCuGU--AUGGGcUGAUGcuu -5' |
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| 20712 | 3' | -54.7 | NC_004688.1 | + | 75459 | 0.68 | 0.808034 |
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Target: 5'- aGUGGCGCuaccGACAccagcCCCGGCcgACGAc -3' miRNA: 3'- aCACCGCG----CUGUau---GGGCUGa-UGCUu -5' |
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| 20712 | 3' | -54.7 | NC_004688.1 | + | 8971 | 0.68 | 0.789423 |
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Target: 5'- gGUcGCGCG-CAUaccacGCCCGACUGgGAAa -3' miRNA: 3'- aCAcCGCGCuGUA-----UGGGCUGAUgCUU- -5' |
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| 20712 | 3' | -54.7 | NC_004688.1 | + | 26465 | 0.7 | 0.678351 |
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Target: 5'- -cUGGCGCaGGCAggggACCCGGCcgacGCGAAu -3' miRNA: 3'- acACCGCG-CUGUa---UGGGCUGa---UGCUU- -5' |
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| 20712 | 3' | -54.7 | NC_004688.1 | + | 71071 | 0.7 | 0.667806 |
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Target: 5'- uUG-GGUGCGGCGUcUgCGACUGCGGGg -3' miRNA: 3'- -ACaCCGCGCUGUAuGgGCUGAUGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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