Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20716 | 5' | -58.9 | NC_004688.1 | + | 36918 | 0.66 | 0.718325 |
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Target: 5'- gUUCCGaGGCcgCCcagCCCUcGGCCGCGAg -3' miRNA: 3'- -AAGGCgCUGa-GGa--GGGAcUUGGCGCU- -5' |
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| 20716 | 5' | -58.9 | NC_004688.1 | + | 88434 | 0.66 | 0.707305 |
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Target: 5'- gUCgGCGAggcggauUUCCUCCCUGGuCUGCu- -3' miRNA: 3'- aAGgCGCU-------GAGGAGGGACUuGGCGcu -5' |
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| 20716 | 5' | -58.9 | NC_004688.1 | + | 37438 | 0.66 | 0.698229 |
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Target: 5'- gUCCcCGACUCCaCCCUGuccaccGAgCGCGGa -3' miRNA: 3'- aAGGcGCUGAGGaGGGAC------UUgGCGCU- -5' |
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| 20716 | 5' | -58.9 | NC_004688.1 | + | 39633 | 0.66 | 0.67791 |
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Target: 5'- -cCCgGCGGCUCUUCCCgcuuCCGCu- -3' miRNA: 3'- aaGG-CGCUGAGGAGGGacuuGGCGcu -5' |
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| 20716 | 5' | -58.9 | NC_004688.1 | + | 57181 | 0.67 | 0.64614 |
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Target: 5'- cUCCGCGACgacggagaacguuUCCU-UCUGGGCCuGCGGg -3' miRNA: 3'- aAGGCGCUG-------------AGGAgGGACUUGG-CGCU- -5' |
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| 20716 | 5' | -58.9 | NC_004688.1 | + | 35290 | 0.67 | 0.636885 |
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Target: 5'- -gCCGCgGACUCCUCCggCUG-ACC-CGAg -3' miRNA: 3'- aaGGCG-CUGAGGAGG--GACuUGGcGCU- -5' |
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| 20716 | 5' | -58.9 | NC_004688.1 | + | 107101 | 0.67 | 0.626598 |
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Target: 5'- uUUCUGCGAC-CCUUCCguuGGCCGCc- -3' miRNA: 3'- -AAGGCGCUGaGGAGGGac-UUGGCGcu -5' |
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| 20716 | 5' | -58.9 | NC_004688.1 | + | 87990 | 0.68 | 0.595795 |
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Target: 5'- -gCCGCGGCgCCUacCCCUaccGCCGCGGc -3' miRNA: 3'- aaGGCGCUGaGGA--GGGAcu-UGGCGCU- -5' |
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| 20716 | 5' | -58.9 | NC_004688.1 | + | 101988 | 0.69 | 0.525171 |
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Target: 5'- -gCCGCG-CUCCaUCUUGA-CCGCGAg -3' miRNA: 3'- aaGGCGCuGAGGaGGGACUuGGCGCU- -5' |
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| 20716 | 5' | -58.9 | NC_004688.1 | + | 106326 | 0.72 | 0.362527 |
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Target: 5'- aUCCGCGGCUCCUgaaCCCccauuaggggcGAGCCGCu- -3' miRNA: 3'- aAGGCGCUGAGGA---GGGa----------CUUGGCGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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