Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20718 | 5' | -53.4 | NC_004688.1 | + | 109161 | 0.66 | 0.928645 |
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Target: 5'- -gCCGCgAAGGcugaGAUCGAGGAUuuGUCGAu -3' miRNA: 3'- agGGCG-UUCCa---CUAGUUCCUG--CAGUU- -5' |
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| 20718 | 5' | -53.4 | NC_004688.1 | + | 10945 | 0.68 | 0.84426 |
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Target: 5'- -aCCGUGGcaGUGggCAAGGACGUCGAg -3' miRNA: 3'- agGGCGUUc-CACuaGUUCCUGCAGUU- -5' |
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| 20718 | 5' | -53.4 | NC_004688.1 | + | 19811 | 0.68 | 0.827815 |
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Target: 5'- -gCCGcCGAGGUGAUCcaccuugccggucuGGACGUCGc -3' miRNA: 3'- agGGC-GUUCCACUAGuu------------CCUGCAGUu -5' |
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| 20718 | 5' | -53.4 | NC_004688.1 | + | 64693 | 0.69 | 0.817967 |
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Target: 5'- gUCCGCccGGGUGcUCcAGGugGUCGAa -3' miRNA: 3'- aGGGCGu-UCCACuAGuUCCugCAGUU- -5' |
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| 20718 | 5' | -53.4 | NC_004688.1 | + | 76867 | 0.69 | 0.799494 |
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Target: 5'- aCCaaCGUggGGggcGUCGGGGGCGUCAAa -3' miRNA: 3'- aGG--GCGuuCCac-UAGUUCCUGCAGUU- -5' |
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| 20718 | 5' | -53.4 | NC_004688.1 | + | 47155 | 0.69 | 0.780354 |
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Target: 5'- cUCCGCcAGGUauUCGAGGGCGUUg- -3' miRNA: 3'- aGGGCGuUCCAcuAGUUCCUGCAGuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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