Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2072 | 3' | -60.3 | NC_001348.1 | + | 123968 | 0.66 | 0.629011 |
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Target: 5'- aGGGAcggagACCGGGGgcCGCCGG-GcCGGAGg -3' miRNA: 3'- cCCCU-----UGGCCCC--GCGGUCuCuGUCUU- -5' |
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| 2072 | 3' | -60.3 | NC_001348.1 | + | 105824 | 0.66 | 0.629011 |
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Target: 5'- aGGGAcggagACCGGGGgcCGCCGG-GcCGGAGg -3' miRNA: 3'- cCCCU-----UGGCCCC--GCGGUCuCuGUCUU- -5' |
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| 2072 | 3' | -60.3 | NC_001348.1 | + | 119336 | 0.67 | 0.578557 |
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Target: 5'- gGGGGAuuCCGGGGCG--GGAGACc--- -3' miRNA: 3'- -CCCCUu-GGCCCCGCggUCUCUGucuu -5' |
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| 2072 | 3' | -60.3 | NC_001348.1 | + | 110456 | 0.67 | 0.578557 |
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Target: 5'- gGGGGAuuCCGGGGCG--GGAGACc--- -3' miRNA: 3'- -CCCCUu-GGCCCCGCggUCUCUGucuu -5' |
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| 2072 | 3' | -60.3 | NC_001348.1 | + | 121940 | 1.07 | 0.001104 |
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Target: 5'- gGGGGAACCGGGGCGCCAGAGACAGAAa -3' miRNA: 3'- -CCCCUUGGCCCCGCGGUCUCUGUCUU- -5' |
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| 2072 | 3' | -60.3 | NC_001348.1 | + | 107852 | 1.07 | 0.001104 |
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Target: 5'- gGGGGAACCGGGGCGCCAGAGACAGAAa -3' miRNA: 3'- -CCCCUUGGCCCCGCGGUCUCUGUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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