Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2072 | 5' | -57.6 | NC_001348.1 | + | 121615 | 0.65 | 0.82979 |
Target: 5'- gCCCUGCCGgGAagaucccacgcaCCAGA--CCCGAUu -3' miRNA: 3'- -GGGACGGCgCUa-----------GGUCUugGGGCUAu -5' |
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2072 | 5' | -57.6 | NC_001348.1 | + | 108177 | 0.65 | 0.82979 |
Target: 5'- gCCCUGCCGgGAagaucccacgcaCCAGA--CCCGAUu -3' miRNA: 3'- -GGGACGGCgCUa-----------GGUCUugGGGCUAu -5' |
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2072 | 5' | -57.6 | NC_001348.1 | + | 106518 | 0.66 | 0.805621 |
Target: 5'- aUCUGCCGC-AUCCAGGcgcACCuCCGu-- -3' miRNA: 3'- gGGACGGCGcUAGGUCU---UGG-GGCuau -5' |
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2072 | 5' | -57.6 | NC_001348.1 | + | 123274 | 0.66 | 0.805621 |
Target: 5'- aUCUGCCGC-AUCCAGGcgcACCuCCGu-- -3' miRNA: 3'- gGGACGGCGcUAGGUCU---UGG-GGCuau -5' |
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2072 | 5' | -57.6 | NC_001348.1 | + | 124005 | 0.67 | 0.740225 |
Target: 5'- -gCUGCCcCGAgccCCAG-ACCCCGAg- -3' miRNA: 3'- ggGACGGcGCUa--GGUCuUGGGGCUau -5' |
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2072 | 5' | -57.6 | NC_001348.1 | + | 110573 | 0.68 | 0.690499 |
Target: 5'- cCCCUgaacgGCCGgGAuacgUCgCGGGGCCCCGGc- -3' miRNA: 3'- -GGGA-----CGGCgCU----AG-GUCUUGGGGCUau -5' |
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2072 | 5' | -57.6 | NC_001348.1 | + | 119219 | 0.68 | 0.690499 |
Target: 5'- cCCCUgaacgGCCGgGAuacgUCgCGGGGCCCCGGc- -3' miRNA: 3'- -GGGA-----CGGCgCU----AG-GUCUUGGGGCUau -5' |
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2072 | 5' | -57.6 | NC_001348.1 | + | 69905 | 0.69 | 0.649786 |
Target: 5'- uUCCgcaaagGUCGCGAUCCAccgcCCCCGAUc -3' miRNA: 3'- -GGGa-----CGGCGCUAGGUcuu-GGGGCUAu -5' |
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2072 | 5' | -57.6 | NC_001348.1 | + | 103945 | 0.69 | 0.649786 |
Target: 5'- aCCUGCUGUucGAU-CGGcGCCCCGGUGg -3' miRNA: 3'- gGGACGGCG--CUAgGUCuUGGGGCUAU- -5' |
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2072 | 5' | -57.6 | NC_001348.1 | + | 73336 | 0.71 | 0.508635 |
Target: 5'- uUCCgggggacGCUGCGGUCCGGGAaggucuacgucCCCCGGUGc -3' miRNA: 3'- -GGGa------CGGCGCUAGGUCUU-----------GGGGCUAU- -5' |
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2072 | 5' | -57.6 | NC_001348.1 | + | 42554 | 0.78 | 0.198056 |
Target: 5'- uCCCUGuuGCGGuuguugUCCAGAagACCCCGAc- -3' miRNA: 3'- -GGGACggCGCU------AGGUCU--UGGGGCUau -5' |
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2072 | 5' | -57.6 | NC_001348.1 | + | 121974 | 1.08 | 0.001888 |
Target: 5'- uCCCUGCCGCGAUCCAGAACCCCGAUAa -3' miRNA: 3'- -GGGACGGCGCUAGGUCUUGGGGCUAU- -5' |
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2072 | 5' | -57.6 | NC_001348.1 | + | 107818 | 1.08 | 0.001888 |
Target: 5'- uCCCUGCCGCGAUCCAGAACCCCGAUAa -3' miRNA: 3'- -GGGACGGCGCUAGGUCUUGGGGCUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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