Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20721 | 3' | -61.1 | NC_004688.1 | + | 82467 | 0.71 | 0.371753 |
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Target: 5'- -uCCGGGAAGCCGGG-CUGGUcG-CCg -3' miRNA: 3'- guGGCUCUUCGGCCCuGGCCGcCaGG- -5' |
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| 20721 | 3' | -61.1 | NC_004688.1 | + | 75381 | 0.72 | 0.333621 |
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Target: 5'- gGCCGAcGggGCUGaGcCCGcGCGGUCCc -3' miRNA: 3'- gUGGCU-CuuCGGCcCuGGC-CGCCAGG- -5' |
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| 20721 | 3' | -61.1 | NC_004688.1 | + | 82670 | 0.72 | 0.333621 |
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Target: 5'- gAUCGGGAAGCCGcugcgcaugacGGuguACUGGCGGUCg -3' miRNA: 3'- gUGGCUCUUCGGC-----------CC---UGGCCGCCAGg -5' |
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| 20721 | 3' | -61.1 | NC_004688.1 | + | 89731 | 0.72 | 0.291752 |
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Target: 5'- cCGCgGAGGcAGUCGGGGCCgagguGGUGGUCa -3' miRNA: 3'- -GUGgCUCU-UCGGCCCUGG-----CCGCCAGg -5' |
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| 20721 | 3' | -61.1 | NC_004688.1 | + | 93426 | 0.73 | 0.260092 |
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Target: 5'- gGCCGGgugaacGAAGUCGGGugCGGUGcUCCa -3' miRNA: 3'- gUGGCU------CUUCGGCCCugGCCGCcAGG- -5' |
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| 20721 | 3' | -61.1 | NC_004688.1 | + | 66614 | 0.74 | 0.242475 |
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Target: 5'- gGCCGcaAGcAGUCGGGGCCGGCGaugCCg -3' miRNA: 3'- gUGGC--UCuUCGGCCCUGGCCGCca-GG- -5' |
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| 20721 | 3' | -61.1 | NC_004688.1 | + | 27957 | 0.78 | 0.125023 |
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Target: 5'- cCACCGAGGuaGGCgGGGACCGGCcaguGG-CCu -3' miRNA: 3'- -GUGGCUCU--UCGgCCCUGGCCG----CCaGG- -5' |
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| 20721 | 3' | -61.1 | NC_004688.1 | + | 67001 | 0.84 | 0.049081 |
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Target: 5'- cCGCCccGAAGCCGGGACCGGCaccGUCCg -3' miRNA: 3'- -GUGGcuCUUCGGCCCUGGCCGc--CAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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