miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
20721 5' -57.9 NC_004688.1 + 93423 0.66 0.769452
Target:  5'- uGCGG-CCGGGUGaacgaagucggguGCGGUgcUCCAGgCGGa -3'
miRNA:   3'- -UGCUaGGCCCAC-------------UGCCGa-AGGUC-GCU- -5'
20721 5' -57.9 NC_004688.1 + 67935 0.66 0.751293
Target:  5'- -gGcgCCGGGUGG-GGaccguucugUCCAGCGAg -3'
miRNA:   3'- ugCuaGGCCCACUgCCga-------AGGUCGCU- -5'
20721 5' -57.9 NC_004688.1 + 26788 0.67 0.741578
Target:  5'- uGCGGUggCCGcGGagcuUGAgGGCUcugaUCCGGCGAu -3'
miRNA:   3'- -UGCUA--GGC-CC----ACUgCCGA----AGGUCGCU- -5'
20721 5' -57.9 NC_004688.1 + 22634 0.67 0.731767
Target:  5'- uACGAggucggCCGGGcUGACGGUgag-GGCGAa -3'
miRNA:   3'- -UGCUa-----GGCCC-ACUGCCGaaggUCGCU- -5'
20721 5' -57.9 NC_004688.1 + 82512 0.67 0.700841
Target:  5'- cCGGaCUGGGUGACGGUggcgUugucgccgaacgaCCAGCGGu -3'
miRNA:   3'- uGCUaGGCCCACUGCCGa---A-------------GGUCGCU- -5'
20721 5' -57.9 NC_004688.1 + 54556 0.68 0.681593
Target:  5'- cCGAuaUCCGaGUcGcCGGCUUCCAGCGc -3'
miRNA:   3'- uGCU--AGGCcCA-CuGCCGAAGGUCGCu -5'
20721 5' -57.9 NC_004688.1 + 106562 0.68 0.630386
Target:  5'- uGCGGUCCaGGGUGuCGGCga--GGCGc -3'
miRNA:   3'- -UGCUAGG-CCCACuGCCGaaggUCGCu -5'
20721 5' -57.9 NC_004688.1 + 71889 0.69 0.599609
Target:  5'- gGCGcgCCGGGgauaacGACGGCc-CCGGCa- -3'
miRNA:   3'- -UGCuaGGCCCa-----CUGCCGaaGGUCGcu -5'
20721 5' -57.9 NC_004688.1 + 86865 0.69 0.599609
Target:  5'- -aGG-CCGGGauacUGACGGCcgCCAGCGc -3'
miRNA:   3'- ugCUaGGCCC----ACUGCCGaaGGUCGCu -5'
20721 5' -57.9 NC_004688.1 + 46605 0.7 0.557935
Target:  5'- gGCGGuuauccaUCCGgucgccauuuaGGUGGCGGCagUCCAGCGu -3'
miRNA:   3'- -UGCU-------AGGC-----------CCACUGCCGa-AGGUCGCu -5'
20721 5' -57.9 NC_004688.1 + 18776 0.72 0.425187
Target:  5'- -gGGUCCGGGUcGAcguauuCGGCcUCCAGCGc -3'
miRNA:   3'- ugCUAGGCCCA-CU------GCCGaAGGUCGCu -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.