Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20725 | 5' | -66.7 | NC_004688.1 | + | 62494 | 0.66 | 0.336377 |
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Target: 5'- cUGGAGCGGCgGGCacaUCGGCCuacauauGGcCCAg -3' miRNA: 3'- -AUCUCGCCG-CCGg--GGCCGG-------CCaGGUa -5' |
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| 20725 | 5' | -66.7 | NC_004688.1 | + | 107744 | 0.66 | 0.322683 |
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Target: 5'- -cGAGCGGCGGCagCgGGCCacGGagCCAUc -3' miRNA: 3'- auCUCGCCGCCGg-GgCCGG--CCa-GGUA- -5' |
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| 20725 | 5' | -66.7 | NC_004688.1 | + | 83317 | 0.66 | 0.315644 |
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Target: 5'- cGGGGUccaGCGGgCCCGGCCGGggaUCAg -3' miRNA: 3'- aUCUCGc--CGCCgGGGCCGGCCa--GGUa -5' |
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| 20725 | 5' | -66.7 | NC_004688.1 | + | 68866 | 0.67 | 0.295227 |
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Target: 5'- -uGGGCGGCGGggUC-GCCGGUCCGg -3' miRNA: 3'- auCUCGCCGCCggGGcCGGCCAGGUa -5' |
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| 20725 | 5' | -66.7 | NC_004688.1 | + | 59497 | 0.67 | 0.275853 |
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Target: 5'- gGGucGCGGUcGCaCCgGGCCGGUCCu- -3' miRNA: 3'- aUCu-CGCCGcCG-GGgCCGGCCAGGua -5' |
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| 20725 | 5' | -66.7 | NC_004688.1 | + | 62617 | 0.69 | 0.20843 |
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Target: 5'- uUGGcGCGGCGGCgaCCGcUCGGUCCAc -3' miRNA: 3'- -AUCuCGCCGCCGg-GGCcGGCCAGGUa -5' |
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| 20725 | 5' | -66.7 | NC_004688.1 | + | 110588 | 0.7 | 0.16764 |
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Target: 5'- -cGGGgGGCGGCCCCGccCCGGuuUCCGg -3' miRNA: 3'- auCUCgCCGCCGGGGCc-GGCC--AGGUa -5' |
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| 20725 | 5' | -66.7 | NC_004688.1 | + | 20974 | 0.73 | 0.115365 |
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Target: 5'- gGGuGCGGCGGCucgccgCCUGGCCGGggUCCGc -3' miRNA: 3'- aUCuCGCCGCCG------GGGCCGGCC--AGGUa -5' |
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| 20725 | 5' | -66.7 | NC_004688.1 | + | 56455 | 0.8 | 0.03404 |
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Target: 5'- cUGGAGUGGUGG-CCCGGCCGGUCUc- -3' miRNA: 3'- -AUCUCGCCGCCgGGGCCGGCCAGGua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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