Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2073 | 3' | -49.9 | NC_001348.1 | + | 51569 | 0.66 | 0.99314 |
Target: 5'- -cUGCCguCGCCCGAGGAGcggcccaccuuGCuuuUGAUGa -3' miRNA: 3'- cuAUGG--GCGGGUUCCUU-----------UGu--ACUACg -5' |
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2073 | 3' | -49.9 | NC_001348.1 | + | 107313 | 0.66 | 0.992069 |
Target: 5'- cGGUACCCGCCCGucccGGugGCAaca-GCu -3' miRNA: 3'- -CUAUGGGCGGGUu---CCuuUGUacuaCG- -5' |
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2073 | 3' | -49.9 | NC_001348.1 | + | 122479 | 0.66 | 0.992069 |
Target: 5'- cGGUACCCGCCCGucccGGugGCAaca-GCu -3' miRNA: 3'- -CUAUGGGCGGGUu---CCuuUGUacuaCG- -5' |
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2073 | 3' | -49.9 | NC_001348.1 | + | 120068 | 0.66 | 0.991723 |
Target: 5'- cGUG-CCGCCUgacauaaucguuggGAGGGGugGUGGUGUa -3' miRNA: 3'- cUAUgGGCGGG--------------UUCCUUugUACUACG- -5' |
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2073 | 3' | -49.9 | NC_001348.1 | + | 109724 | 0.66 | 0.991723 |
Target: 5'- cGUG-CCGCCUgacauaaucguuggGAGGGGugGUGGUGUa -3' miRNA: 3'- cUAUgGGCGGG--------------UUCCUUugUACUACG- -5' |
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2073 | 3' | -49.9 | NC_001348.1 | + | 121358 | 0.67 | 0.982595 |
Target: 5'- --gGCCCGCUCAGGGAAagaGCccGAa-- -3' miRNA: 3'- cuaUGGGCGGGUUCCUU---UGuaCUacg -5' |
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2073 | 3' | -49.9 | NC_001348.1 | + | 108434 | 0.67 | 0.982595 |
Target: 5'- --gGCCCGCUCAGGGAAagaGCccGAa-- -3' miRNA: 3'- cuaUGGGCGGGUUCCUU---UGuaCUacg -5' |
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2073 | 3' | -49.9 | NC_001348.1 | + | 61269 | 0.68 | 0.975455 |
Target: 5'- --gGCCCGCCgGGGuGGAAUGUGgcGUu -3' miRNA: 3'- cuaUGGGCGGgUUC-CUUUGUACuaCG- -5' |
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2073 | 3' | -49.9 | NC_001348.1 | + | 73879 | 0.69 | 0.959154 |
Target: 5'- --gACCCGUUCGGGGAGACAcgGGUc- -3' miRNA: 3'- cuaUGGGCGGGUUCCUUUGUa-CUAcg -5' |
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2073 | 3' | -49.9 | NC_001348.1 | + | 106861 | 0.73 | 0.829793 |
Target: 5'- gGAUACCCGCCUcuuggccuuGGAAaccACAUGAucgucUGCa -3' miRNA: 3'- -CUAUGGGCGGGuu-------CCUU---UGUACU-----ACG- -5' |
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2073 | 3' | -49.9 | NC_001348.1 | + | 122930 | 0.73 | 0.829793 |
Target: 5'- gGAUACCCGCCUcuuggccuuGGAAaccACAUGAucgucUGCa -3' miRNA: 3'- -CUAUGGGCGGGuu-------CCUU---UGUACU-----ACG- -5' |
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2073 | 3' | -49.9 | NC_001348.1 | + | 121527 | 1.12 | 0.005882 |
Target: 5'- uGAUACCCGCCCAAGGAAACAUGAUGCc -3' miRNA: 3'- -CUAUGGGCGGGUUCCUUUGUACUACG- -5' |
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2073 | 3' | -49.9 | NC_001348.1 | + | 108265 | 1.12 | 0.005882 |
Target: 5'- uGAUACCCGCCCAAGGAAACAUGAUGCc -3' miRNA: 3'- -CUAUGGGCGGGUUCCUUUGUACUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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