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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2073 | 5' | -55.1 | NC_001348.1 | + | 85338 | 0.66 | 0.900953 |
Target: 5'- gGACuuauaggCGCGUCgUUGGACGUUCu -3' miRNA: 3'- aUUGugga---GCGCAGgGACCUGCAAGc -5' |
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2073 | 5' | -55.1 | NC_001348.1 | + | 47171 | 0.67 | 0.857924 |
Target: 5'- uUAACAaCUCGUGUCUCUGGAauaaaaCGUUUu -3' miRNA: 3'- -AUUGUgGAGCGCAGGGACCU------GCAAGc -5' |
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2073 | 5' | -55.1 | NC_001348.1 | + | 67948 | 0.69 | 0.770259 |
Target: 5'- cAAUACCUCGUuaagUCCUGGGCGUgUUGg -3' miRNA: 3'- aUUGUGGAGCGca--GGGACCUGCA-AGC- -5' |
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2073 | 5' | -55.1 | NC_001348.1 | + | 121561 | 1.07 | 0.003985 |
Target: 5'- aUAACACCUCGCGUCCCUGGACGUUCGu -3' miRNA: 3'- -AUUGUGGAGCGCAGGGACCUGCAAGC- -5' |
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2073 | 5' | -55.1 | NC_001348.1 | + | 108231 | 1.07 | 0.003985 |
Target: 5'- aUAACACCUCGCGUCCCUGGACGUUCGu -3' miRNA: 3'- -AUUGUGGAGCGCAGGGACCUGCAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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