Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20735 | 5' | -55.3 | NC_004688.1 | + | 65781 | 0.66 | 0.851224 |
Target: 5'- cGGUGGUGGCGGCCuGCUUagcguuguggCGCCg--- -3' miRNA: 3'- -UCACCGUUGUCGG-CGAAa---------GCGGacuu -5' |
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20735 | 5' | -55.3 | NC_004688.1 | + | 90470 | 0.66 | 0.825419 |
Target: 5'- aGGUGGUAGaagccgaGGCCGUUaUCGCCa--- -3' miRNA: 3'- -UCACCGUUg------UCGGCGAaAGCGGacuu -5' |
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20735 | 5' | -55.3 | NC_004688.1 | + | 34920 | 0.67 | 0.768836 |
Target: 5'- uGGUGGC--CGG-CGCUUcUCGCCUGGc -3' miRNA: 3'- -UCACCGuuGUCgGCGAA-AGCGGACUu -5' |
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20735 | 5' | -55.3 | NC_004688.1 | + | 45884 | 0.67 | 0.74879 |
Target: 5'- -aUGGCGGCAGCgGCcacCGCCgcgGAAu -3' miRNA: 3'- ucACCGUUGUCGgCGaaaGCGGa--CUU- -5' |
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20735 | 5' | -55.3 | NC_004688.1 | + | 92306 | 0.68 | 0.738593 |
Target: 5'- cAGUGGCccCGGCCGCcaucacCGCCgGAAc -3' miRNA: 3'- -UCACCGuuGUCGGCGaaa---GCGGaCUU- -5' |
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20735 | 5' | -55.3 | NC_004688.1 | + | 20970 | 0.75 | 0.357173 |
Target: 5'- gGGUGGguGCGGCgGCUcgcCGCCUGGc -3' miRNA: 3'- -UCACCguUGUCGgCGAaa-GCGGACUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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