Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20737 | 3' | -61.1 | NC_004688.1 | + | 49866 | 0.66 | 0.648523 |
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Target: 5'- cGGCcGaGgCCCCGCCGACggccagGAAGUc-- -3' miRNA: 3'- -CCGaC-CgGGGGCGGCUGa-----CUUCAccc -5' |
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| 20737 | 3' | -61.1 | NC_004688.1 | + | 79823 | 0.66 | 0.648523 |
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Target: 5'- cGUUGguGCCCCCGCCGagGCUGgcGaGGu -3' miRNA: 3'- cCGAC--CGGGGGCGGC--UGACuuCaCCc -5' |
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| 20737 | 3' | -61.1 | NC_004688.1 | + | 60645 | 0.66 | 0.6285 |
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Target: 5'- cGCUGaGUgCaCCGCCGACUGGccGGUGc- -3' miRNA: 3'- cCGAC-CGgG-GGCGGCUGACU--UCACcc -5' |
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| 20737 | 3' | -61.1 | NC_004688.1 | + | 31020 | 0.66 | 0.598502 |
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Target: 5'- ---gGGCgCCCGUCGGCcGA-GUGGGc -3' miRNA: 3'- ccgaCCGgGGGCGGCUGaCUuCACCC- -5' |
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| 20737 | 3' | -61.1 | NC_004688.1 | + | 10206 | 0.66 | 0.598502 |
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Target: 5'- cGGC-GGCaacaaUCGCCGuGCUGAcGUGGGu -3' miRNA: 3'- -CCGaCCGgg---GGCGGC-UGACUuCACCC- -5' |
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| 20737 | 3' | -61.1 | NC_004688.1 | + | 46685 | 0.67 | 0.558866 |
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Target: 5'- aGGC-GGugaaCCCCCGCCGACcaaaugGAauGGUcGGGu -3' miRNA: 3'- -CCGaCC----GGGGGCGGCUGa-----CU--UCA-CCC- -5' |
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| 20737 | 3' | -61.1 | NC_004688.1 | + | 41131 | 0.7 | 0.402629 |
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Target: 5'- cGGCUugucGCCCUCGCacuCGA-UGGAGUGGGa -3' miRNA: 3'- -CCGAc---CGGGGGCG---GCUgACUUCACCC- -5' |
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| 20737 | 3' | -61.1 | NC_004688.1 | + | 44706 | 0.79 | 0.114616 |
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Target: 5'- aGGCUGGCCCCUGUCGAUauccaGggGcuUGGGc -3' miRNA: 3'- -CCGACCGGGGGCGGCUGa----CuuC--ACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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