miRNA: 3'- cUugCUACCC--------CUUUGCCCGUCUCCau -5'
miRNA: 3'- -CUUGCU----ACCCCUU---UGCCCgUCUCCau -5'
miRNA: 3'- -CUUGCU----ACCCCUU---UGCCCgUCUCCau -5'
miRNA: 3'- -CUUGCUACCCCUUUGCCCGUCUCCAU- -5'
miRNA: 3'- -CUUGCUACCCCUUUGCCCGUCUCCAU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2074 | 3' | -54.6 | NC_001348.1 | + | 104665 | 0.7 | 0.731849 |
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Target: 5'- aGugGGUGGGacuuaaaaGAAAUGGGUGGAGGg- -3' miRNA: 3'- cUugCUACCC--------CUUUGCCCGUCUCCau -5' |
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| 2074 | 3' | -54.6 | NC_001348.1 | + | 105275 | 0.77 | 0.349081 |
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Target: 5'- aGGACGAugugUGGGGAGggcGCGGGgGGAGGa- -3' miRNA: 3'- -CUUGCU----ACCCCUU---UGCCCgUCUCCau -5' |
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| 2074 | 3' | -54.6 | NC_001348.1 | + | 124517 | 0.77 | 0.349081 |
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Target: 5'- aGGACGAugugUGGGGAGggcGCGGGgGGAGGa- -3' miRNA: 3'- -CUUGCU----ACCCCUU---UGCCCgUCUCCau -5' |
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| 2074 | 3' | -54.6 | NC_001348.1 | + | 121079 | 1.06 | 0.004325 |
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Target: 5'- uGAACGAUGGGGAAACGGGCAGAGGUAc -3' miRNA: 3'- -CUUGCUACCCCUUUGCCCGUCUCCAU- -5' |
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| 2074 | 3' | -54.6 | NC_001348.1 | + | 108713 | 1.06 | 0.004325 |
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Target: 5'- uGAACGAUGGGGAAACGGGCAGAGGUAc -3' miRNA: 3'- -CUUGCUACCCCUUUGCCCGUCUCCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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